Synthesis and characterization of derivative of 1,10-phenanthroline, 2,9-bis(aminomethyl)-1,10-phenanthroline (PDAM) and its complex with Ru(II). Study of their interaction with CT-DNA. In vitro exploration of their biological activity.

Postgraduate Thesis uoadl:2883743 229 Read counter

Unit:
Κατεύθυνση Ανόργανη Χημεία και Εφαρμογές της στη Βιομηχανία
Library of the School of Science
Deposit date:
2019-10-29
Year:
2019
Author:
Pappa Rafailia
Supervisors info:
Κωνσταντίνος Μεθενίτης, Αναπληρωτής Καθηγητής, Τμήμα Χημείας, Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών
Χριστίνα-Άννα Μητσοπούλου, Καθηγήτρια, Τμήμα Χημείας, Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών
Ελένη Ευθυμιάδου, Επίκουρη Καθηγήτρια, Τμήμα Χημείας, Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών
Original Title:
Σύνθεση και χαρακτηρισμός του παραγώγου της 1,10-φαινανθρολίνης, 2,9-δι(αμινομέθυλο)-1,10-φαινανθρολίνη (PDAM) και του συμπλόκου του Ru(II) με την PDAM. Μελέτη της αλληλεπίδρασης αυτών με CT-DNA. In vitro διερεύνηση της βιολογικής τους δράσης.
Languages:
Greek
Translated title:
Synthesis and characterization of derivative of 1,10-phenanthroline, 2,9-bis(aminomethyl)-1,10-phenanthroline (PDAM) and its complex with Ru(II). Study of their interaction with CT-DNA. In vitro exploration of their biological activity.
Summary:
The aim of the present thesis is the synthesis and characterization of Ru(II) complex with derivative of 1,10-phenanthroline, study of their interaction with CT-DNA and in vitro exploration of their biological activity as a potential new anticancer agent. The corresponding Ru(II) complex was successfully synthesized and fully characterized by various spectroscopic methods (IR, Fluorescence, NMR, MS, UV-Vis, CD). In addition, their binding to CT-DNA was also been evaluated by Circular dichroism (CD), UV-Vis Spectroscopy, Fluorescence and Viscometry. The data reveal that both the ligand and the complex strongly interact with CT-DNA. The PDAM ligand was found to interact with CT-DNA via intercalation (classical or non-classical). On the other hand, the complex appears to interact in more than one way and the types of interaction depend on the ratio R=[drug]/[DNA] where [drug]:[Ru(II)-PDAM]. So, some kind of intercalation is proposed by simultaneously converting of B structure of DNA into A. In terms of cytotoxicity, both the unblocked PDAM and the Ru(II)-PDAM complex showed a significant toxicity activity in human MCF-7 and U87MG tumor cell lines, whereas fluorescence microscopy results in both MCF-7 cell lines, as in U87MG were particularly encouraging with fluorescence in U87MG cells being more pronounced, possibly indicating selectivity for the particular cell type.
Main subject category:
Science
Keywords:
1,10-phenanthroline, ruthenium(II), DNA, anticancer activity
Index:
No
Number of index pages:
0
Contains images:
Yes
Number of references:
99
Number of pages:
163
File:
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