Μετατροπή μεσεγχυματικών κυττάρων σε πολυδύναμα βλαστοκύτταρα

Postgraduate Thesis uoadl:2886083 279 Read counter

Unit:
Κατεύθυνση Ιατρική Γενετική: Κλινική και Εργαστηριακή Κατεύθυνση
Library of the School of Health Sciences
Deposit date:
2019-12-02
Year:
2019
Author:
Mertzanian Anny
Supervisors info:
Μαρία Τζέτη, Αναπληρώτρια Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ, Επιβλέπουσα
Ιωάννα Traeger - Συνοδινού, Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
Ελένη Φρυσίρα, Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
Original Title:
Μετατροπή μεσεγχυματικών κυττάρων σε πολυδύναμα βλαστοκύτταρα
Languages:
Greek
Translated title:
Μετατροπή μεσεγχυματικών κυττάρων σε πολυδύναμα βλαστοκύτταρα
Summary:
Stem cells are undifferentiated cells characterized by the ability of selfrenewal, pluripotency and in vivo reconstitution of functional tissues. They
come from the early stages of zygote formation and can give birth to all
tissues of the human body. Specifically in the category of pluripotent stem
cells are embryonic stem cells (ESCs) that come from the internal mass of the
blastocyst and are able to differentiate into the tissues of all 3 germinal skins.
Unlimited undifferentiated reproductive potential as well as their ability to
differentiate has been an important tool in the field of research and
regenerative medicine, but it is confronted with moral barriers and dilemmas.
The solution to this comes from induced pluripotent stem cells, where in 2006
Takahasi and Yamanaka succeeded in inducing somatic cell pluripotency
using pluripotent transcription factors and opening new horizons for research
and personalized medicine. The characteristics of iPSCs that resemble those
of ESCs signal their widespread use in several directions: disease modeling
(Disease - specific iPSCs), drug study and future gene and cell therapy.
The purpose of this study was to develop specific iPSCs for DOCK8
Immunodeficiency Syndrome using a reprogramming method with synthetic
mRNA molecules that express the transcription factors OCt4, Sox2, Klf4,
Lin28, c -Myc, in mesenchymal stem cells (BM cells). - MSCs) a patient with
this syndrome, who had a 4-base homozygous deficiency. Successful
reprogramming of BM - MSCs and creation of DOCK8iPSCs with 0.4% yield
was performed. The DOCK8iPSCs were successfully tested for their
identification, pluripotency, and genetic stability.
Evaluating the results of this thesis was the first time that, according to
existing literature, pluripotent, genetically stable and disease-specific iPSCs
for the DOCK8 Immunodeficiency Syndrome were established with a safe and
high-yield method of cell reprogramming, laying the foundations for the study
of the method and planning, with the necessary further studies, possible
therapies.
Main subject category:
Health Sciences
Keywords:
Cell Reprogramming, Disease-specific iPSCs, Synthetic mRNA, DOCK8 Immunodeficiency Syndrome, BM - MSCs
Index:
No
Number of index pages:
0
Contains images:
Yes
Number of references:
98
Number of pages:
101
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