DAZL, OCT4 and Oxytocin Reseptor expression in amniotic fluid- Innovative Culture

Postgraduate Thesis uoadl:2886995 437 Read counter

Unit:
Κατεύθυνση Αναπαραγωγική-Αναγεννητική Ιατρική
Library of the School of Health Sciences
Deposit date:
2019-12-06
Year:
2019
Author:
Platania Varvara
Supervisors info:
Πέτρος Δρακάκης, Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Κωνσταντίνος Στεφανίδης, Αναπληρωτής Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ, Επιβλέπων
Αικατερίνη Ντόμαλη, Επίκουρη Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
Original Title:
Έκφραση DAZL, OCT4 και Υποδοχέα Ωκυτοκίνης σε καινοτόμα καλλιέργεια αμνιακού υγρού
Languages:
Greek
Translated title:
DAZL, OCT4 and Oxytocin Reseptor expression in amniotic fluid- Innovative Culture
Summary:
Human mesenchymal stem cells (MSCs) constitute a multipotent cell population and are able to differentiate into various lineages of mesenchymal tissues. Studies have indicated the capability of MSCs for in vitro regeneration of their tissue of origin. It is evident that MSCs play an important role in the systematic application of autologous cell and gene therapy. Amniotic fluid (AF) represents a noncontroversial MSCs source.
The present study, focused on an innovative technique of cultured AF, in which fetal bovine serum is replaced with amniotic fluid serum, to achieve the identification, isolation and characterization of AF stem cell like cells.
Cells from human amniotic fluid were cultured and AF-MSCs like cells were identified with microscopy. Cells were isolated and analyzed for gene expression of pluripotency markers Oct-4, DAZL and oxytocin receptor.
RT-PCR results indicated high expression of Oct-4 and a significant expression of DAZL and oxytocin receptor.
The expression of pluripotency marker Oct-4 indicates the presence of AF-MSCs like cells suitable for direct use in regenerative medicine due to the fact of animal origin free serum. DAZL expression indicates the presence of germ cells like cells. Oxytocin receptors are expressed in early mammalian developmental stages, suggesting that oxytocin may be involved in the differentiation of stem cells into the germ cell lines at the very early stages of the mammalian development.
Main subject category:
Health Sciences
Keywords:
Amniotic fluid, Stem cells, Pluripotency, Germ cells, Oct4, DAZL, Oxytocin, Reproductive, Regenerative, Medicine
Index:
No
Number of index pages:
0
Contains images:
Yes
Number of references:
187
Number of pages:
111
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