The Participation of miRNAs in Erythropoiesis and Haemoglobin Gene Expression

Postgraduate Thesis uoadl:2897322 22 Read counter

Unit:
Κατεύθυνση Ιατρική Γενετική: Κλινική και Εργαστηριακή Κατεύθυνση
Library of the School of Health Sciences
Deposit date:
2020-02-13
Year:
2020
Author:
Thomadakis Panagiotis
Supervisors info:
Ιωάννα-Ραχήλ Συνοδινού-Traeger, Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ, Επιβλέπουσα
Χριστίνα Βρεττού, Μέλος Ε.ΔΙ.Π., Ιατρική Σχολή, ΕΚΠΑ
Κυριακή Κέκου, Μέλος Ε.ΔΙ.Π., Ιατρική Σχολή, ΕΚΠΑ
Original Title:
Η Συμμετοχή των miRNA στην Ερυθροποίηση και την έκφραση των γονιδίων Αιμοσφαιρίνης
Languages:
Greek
Translated title:
The Participation of miRNAs in Erythropoiesis and Haemoglobin Gene Expression
Summary:
This dissertation was conducted under the aegis of the National and Kapodistrian University of Athens, as part of the Master’s Program “Medical Genetics: Clinical and Laboratory Direction” for the academic year 20192020. It is a bibliographic dissertation which focuses on the analysis and comparison of existing literature with regards to the contribution of miRNAs in erythropoiesis and expression of hemoglobin genes. Firstly, the necessary introductory information is presented, concerning erythropoiesis and hemoglobin’s gene sequence, discussing the various molecules and other factors which contribute to the biogenesis and function of miRNAs. Secondly, all in silico and in vitro methods of miRNA processing are presented, thoroughly explicating both the qPCR method and the various miRNA databases. In the third segment of the present work, the miRNAs that contribute to the procedure of the hematopoiesis, and more specifically that of erythropoiesis, are presented along with the most representative miRNAs connected to the hemoglobin genes and their pathological variants. Thus, miRNAs such as miR150, miR-223, miR-15a and miR-451, which are related both with erythropoiesis and hemoglobin genes are of great importance. Lastly, there is brief mention of pathological cases, such as β-thalassemia, and conclusions regarding the function of miRNAs in research, diagnosis and therapy are drawn, along with hypotheses about their future contribution.
Main subject category:
Health Sciences
Keywords:
miRNAs, Erythropoiesis, Hemoglobin, Gene expression
Index:
Yes
Number of index pages:
3
Contains images:
Yes
Number of references:
161
Number of pages:
109
ΘΩΜΑΔΑΚΗΣ Η ΣΥΜΜΕΤΟΧΗ ΤΩΝ miRNA ΣΤΗΝ ΕΡΥΘΡΟΠΟΙΗΣΗ ΚΑΙ ΤΗΝ ΕΚΦΡΑΣΗ ΤΩΝ ΓΟΝΙΔΙΩΝ ΑΙΜΟΣΦΑΙΡΙΝΗΣ.pdf (2 MB) Open in new window