Κατεύθυνση Περιβάλλον και Υγεία: Διαχείριση Περιβαλλοντικών Θεμάτων με Επιπτώσεις στην Υγεία
Library of the School of Health Sciences

2020-07-07

2020

Politakis Nektarios

Νικόλαος Καβαντζάς (Επιβλέπων), Καθηγητής, Ιατρικής Σχολής , ΕΚΠΑ
Ανδρέας Χ. Λάζαρης, Καθηγητής, Ιατρικής Σχολής, ΕΚΠΑ
Γεώργιος Αγρογιάννης, Αναπληρωτής Καθηγητής, Ιατρικής Σχολής, ΕΚΠΑ

Η ανοσοϊστοχημική μελέτη της Κερατίνης 5/6 σε ουροθηλιακά καρκινώματα της ουροδόχου κύστης: συσχέτιση με περιβαλλοντικούς παράγοντες κινδύνου, την πρόγνωση και ο ρόλος στη μοριακή ταξινόμηση των καρκινωμάτων

Greek

Immunohistochemical study of KRT5/6 expression in bladder urothelial carcinoma: correlation with environmental risk factors, prognosis and the role of KRT5/6 in molecular taxonomy

Κeratin 5/6 (KRT5/6) or Cytokeratin 5/6 (CK5/6) is an epithelial protein which has a significant biological role for cellular differentiation and apoptosis. Its expression is used nowadays for the molecular taxonomy of tumors in breast cancer, since it is a common biomarker of the basal molecular subtype. In the same spectrum, the expression of CK5/6 in urothelial carcinoma of bladder is considered a potent biomarker for the molecular taxonomy of the tumors and represents the basal subtype. The use of expression of CK5/6 is of great prognostic and strategic value for the study of the progression and the best therapeutic plan. This study examines the expression of CK5/6 in muscle invasive and non-muscle invasive urothelial carcinomas of bladder in correlation with the classic clinicopathological and anatomical parameters (age, gender, grade and stage). The aim of this study is to designate the value of CK5/6 as a prognostic biomarker and appoint its use for the characterisation of tumors as of basic subtype in molecular taxonomy. The expression of CK5/6 was found with immunohistochemical (IHC) method (polymer antibody bound with Horse Radish Peroxydase) in paraffin embedded tissue sections, 4nm thick, from 120 patients. Ιn the total pool, it was observed positive expression of CK5/6 in 101 tumors (84.2%) from which the 48 (40%) showed focal positive expression and the 53 (44.2%) showed positive expression. The univariate statistical analysis of the results showed positive correlation between the expression of CK5/6 (% of area stained) and the stage (pTa,pT1,pT2) (p=0.037). The same positive correlation was found between the expression of CK5/6 (% of area stained) and the stage of muscle invasion of the tumors (pTa/pT1, pT2). A positive correlation was also found between the expression of CK5/6 and grade (WHO 1973). From the evalution of the intensity of the CK5/6 IHC stain, it was found that: a) there is a positive correlation between the intensity of the stain and the stage (pTa,pT1,pT2) (p=0.001), b) there is a positive correlation between the intensity of the stain and the muscle invasion (pTa/pT1, pT2) (p=0.003) and c) there is positive correlation between the intensity of the stain and the grade (WHO 1973) (p<0.001). In conclusion, through the correlation of CK5/6 with stage, muscle invasion and grade, it was found that the expression of CK5/6 relates to poor prognosis, tumor aggression and progression in compliance with the latest bibliography. This research also concludes that the expression of CK5/6 can be used combined with other molecular biomarkers for the characterization of the basal molecular subtype in molecular taxonomy of the tumors in bladder.

Health Sciences

Urothelial carcinoma, Bladder, Keratin 5/6, Molecular biomarker, Molecular taxonomy, Basal subtype, Stage, Grade, Immunohistochemical stain, Prognostic marker

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https://pergamos.lib.uoa.gr/uoa/dl/object/2917955