The expression of h-TERT to the cells of amniotic fluid and the cells of endometrium after cultivation

Postgraduate Thesis uoadl:2924203 176 Read counter

Unit:
Κατεύθυνση Αναπαραγωγική-Αναγεννητική Ιατρική
Library of the School of Health Sciences
Deposit date:
2020-10-07
Year:
2020
Author:
Vasileiou Georgia
Supervisors info:
Στεφανίδης Κωνσταντίνος, Αναπληρωτής Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Δρακάκης Πέτρος, Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Μαυρογιάννη Δέσποινα, ΕΔΙΠ, Ιατρική Σχολή, ΕΚΠΑ
Original Title:
Η έκφραση του h-TERT στα κύτταρα του αμνιακού υγρού και στα κύτταρα του ενδομητρίου μετά από καλλιέργεια
Languages:
Greek
Translated title:
The expression of h-TERT to the cells of amniotic fluid and the cells of endometrium after cultivation
Summary:
The amniotic fluid (AF) has been knowledged as an important fluid because in this have been isolated Stem Cells (Amniotic Fluid Stem Cells –AFCs), those cells show high plasticity, self – renewal and the capacity to differentiate into Nerve cells, Osteoblasts cells and Adipoce cells. AFCs, primarily, have been suggested to be categorized as Multipotent Stem Cells but nevertheless they express some genes of Pluripotent Stem Cells (Embryonic Stem Cells) and mainly in germ stem cell line as CD117 (c-kit), something very important and highly promising for the future. These markers are OCT 3/4, SSEA-4, SOX-2, NANOG, C-myc and CD117(C-kit), which has been mentioned. The h-TERT gene (5p15.33) is the gene of expression human telomerase reverse transcriptase. Telomerase is the enzyme responsible for maintenance of the length of telomeres at the end of chromosomes by addition of repetitive sequences as TTAGGG. The h-TERT expression has been exhibited in Embryonic Stem Cells, Primordial Germ Cells and Cancer Stem Cells as result to be the key of organ and tissue regeneration. So, the expression of this gene in AFCs is very significant because antibody-forming cells (AFCs) express the gene h-TERT at different levels with the the higher transcript levels of expressing the h-TERT protein that is present in the nuclear and cytoplasmic compartment, where the cytosolic h-TERT is embodied in the Extracellular vesicles (EVs) that AFCs secrete in the extracellular mileu. Menstrual - blood derived Stem Cells – MenSCs are Endometrial Mesenchymal Regenerative Cells are capable of differentiating into nine lineages, as Cardiomyocytic, Respiratory Epithelial, Neurocytic, Myocytic, Endothelial, Pancreatic, Hepatic, Adipocytic and Osteogenic. Recently, MenSCs have been known that they show some markers of Embryonic Pluripotent Stem Cells as Oct-4, S0X-2, και SSEA-4, something also promising in Regenerative Medicine.

Main subject category:
Health Sciences
Keywords:
Telomerase, Stem cells, Amniotic fluid, Pluripotent Stem cells, Multipotent Stem cells
Index:
No
Number of index pages:
0
Contains images:
Yes
Number of references:
168
Number of pages:
139
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