Detection and partial characterization of endotoxins in bacteria thriving in pharmaceutical industry.

Postgraduate Thesis uoadl:2926250 55 Read counter

Unit:
Κατεύθυνση Μικροβιακή Βιοτεχνολογία
Library of the School of Science
Deposit date:
2020-10-26
Year:
2020
Author:
Fotopoulou Emmanouela
Supervisors info:
Καραγκούνη-Κύρτσου Αμαλία, Ομότιμη Καθηγήτρια Τμήματος Βιολογίας ΕΚΠΑ
Original Title:
Ανίχνευση και μερικός χαρακτηρισμός ενδοτοξινών σε βακτήρια που ευδοκιμούν σε χώρο Φαρμακοβιομηχανίας.
Languages:
Greek
Translated title:
Detection and partial characterization of endotoxins in bacteria thriving in pharmaceutical industry.
Summary:
Endotoxins are the main component of the outer membrane of the Gram-negative bacteria. They are composed of carbohydrates, fatty acids and phosphate. Endotoxins, which are also known as Lipopolysaccharide (LPS), amongst all the different types and stems of microbes, can vary based on the type of carbohydrates and fatty acids from which are composed. They are the determining factor to the transmission of infections, through commercially available proteins or biologically effective substances. It is possible for the pharmaceuticals industry, for endotoxins to be found in the line of the production of the medicine, as well as the final product itself. An endotoxin can cause a series of patho-physiological reactions, such as fever, endotoxin shock, tissue damage, and death.
The aim of this research is the development and the adaptation of the proper methodology so as to detect and characterize the endotoxins of Gram-negative bacteria in the line of production of pharmaceuticals.
Main subject category:
Science
Keywords:
Endotoxins, Limulus Amebocyte Lysate (LAL), Extraction and Purification, SDS-PAGE
Index:
No
Number of index pages:
0
Contains images:
Yes
Number of references:
64
Number of pages:
83
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