Detection of let-7b and let-7c genes in endometriosis

Postgraduate Thesis uoadl:2928577 34 Read counter

Κατεύθυνση Αναπαραγωγική-Αναγεννητική Ιατρική
Library of the School of Health Sciences
Deposit date:
Polia Afroditi
Supervisors info:
Δρακάκης Πέτρος, Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ

Ντόμαλη Αικατερίνη, Επίκουρη Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ

Μαυρογιάννη Δέσποινα, Ε.ΔΙ.Π, Ιατρική Σχολή, ΕΚΠΑ
Original Title:
Ανίχνευση των γονιδίων let-7b και let-7c στην ενδομητρίωση
Translated title:
Detection of let-7b and let-7c genes in endometriosis
Subfertility is nowadays a scourge affecting a large proportion of couples who wish to have children and it may be due to a factor either on the male or on the female side. Endometriosis is one of the most enigmatic pathological conditions of the female reproductive system and appears to be directly involved in subfertility of women of reproductive age. The factors aiding the survival and subsequent implantation of ectopic endometrium into other tissues remain unknown. Therefore, the difficulty of differential diagnosis of this illness and its distinction from other pathological conditions, combined with the paucity of data regarding its pathophysiology, have made it essential to control its research and clinical aspects. In order to study the molecular mechanisms underlying the appearance of endometriosis, those genes were sought which are involved in cellular functions like multiplication, apoptosis and migration.
In recent years, the miRNAs have been greatly attracting the interest of researchers in subfertility. These non-codifying RNAs have various applications, cancer, the polycystic ovary syndrome and endometriosis being but a few examples that justify the insistence of researchers in further researching subfertile or non-subfertile populations. This MCs work concentrates on one of the most significant gene families of miRNAs, the family let-7. The family of let-7 miRNAs has been involved in the control of various cellular processes and the pathogeny of illnesses. Applications of genes from the let-7 family are also found in the sector of aided reproduction where efforts are made to find a correlation between a gene and endometriosis causing subfertility. This work aims to study the correlation between the expression of the let-7b and let-7c genes with the appearance of endometriosis. For this reason, the samples used were taken from women with a history of endometriosis and the samples included tissue from both ectopic and eutopic endometrium. By using molecular methods in 17 samples from women with endometriosis, we observed a model of low expression in the ectopic endometrium of these women
Main subject category:
Health Sciences
Endometriosis, miRNA, let-7b, let-7c
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