Unit:
Faculty of MedicineLibrary of the School of Health Sciences
Author:
Flesiopoulou Ioanna
Dissertation committee:
Κωνσταντόπουλος Κωνσταντίνος, Καθηγητής, Τμήμα Ιατρικής, ΕΚΠΑ
Πολίτου Μαριάννα, Αναπληρώτρια Καθηγήτρια, Τμήμα Ιατρικής, ΕΚΠΑ
Τσαντές Αργύριος, Αναπληρωτής Καθηγητής, Τμήμα Ιατρικής, ΕΚΠΑ
Γιαλεράκη Αργυρή, Αναπληρώτρια Καθηγήτρια, Τμήμα Ιατρικής, ΕΚΠΑ
Βαλσάμη Σερένα, Επίκουρη Καθηγήτρια, Τμήμα Ιατρικής, ΕΚΠΑ, Επιβλέπουσα
Κοκόρη Στυλιανή, Επίκουρη Καθηγήτρια, Τμήμα Ιατρικής, ΕΚΠΑ
Πιτταράς Θεόδωρος, Επίκουρος Καθηγητής, Τμήμα Ιατρικής, ΕΚΠΑ
Original Title:
Μελέτη αλλοανοσοποίησης ασθενών από μετάγγιση και κύηση
Translated title:
Alloimmunization study in transfused and pregnant patients
Summary:
Red blood cell (RBC) alloantibody titration is a quasi-quantitative method to assess antibody concentration and is considered a useful means of estimating maternal alloimmunization during pregnancy. Traditionally titration is performed using conventional tube test (CTT). Gel microcolumn agglutination based method (GMA) has been proven reliable for many immunohematology tests. Our study compared CTT with GMA of two different commercial available GMA systems for RBC alloantibody titration.
Serum samples with significant RBC-alloantibodies, were evaluated in our study. Each sample was titrated concurrently with CTT, with ID-DiaMed-GmbH, Cressier, Switzerland (GMA1) and with DG Gel Coombs Diagnostic Grifols, Passeig Fluvial, Spain (GMA2).
One hundred thirty seven titrations tests concerning 50 anti-D, 25 anti-Kell, 10 anti-E, 9 anti-Jka, 8 anti-c, 5 anti-Cw, 5 anti-Fya, 7 anti-M, 6 anti-Kpa, 3 anti-Lua, 1 anti-e, 3 anti-G, and 2 anti-Cha were performed and evaluated. Samples tested by CTT versus GMA1 and GMA2 generated mostly equal or higher titers by GMAs. The results of both comparisons were in good agreement (W=0.91, p<0.0001, and W=0.92, p<0.0001 respectively). For all antibodies specificities the mean absolute difference in titers ranged from 1-3 for both GMA1 and GMA2 versus CTT. Samples tested by GMA1 vs GMA2 were in almost perfect agreement (W=0.95, p<0.0001).
Conclusion: Although both GMAs were found slightly more sensitive than CTT for alloantibody titration, the differences were not significant and the agreement between all methods was very good, possibly indicating GMA as a suitable alternative to CTT in RBC antibody titration.
Main subject category:
Health Sciences
Keywords:
Red blood cell antibody titration, Alloimmunization, Titration method
Number of references:
364
