Κατεύθυνση Κλινική Βιοχημεία - Μοριακή Διαγνωστική
Library of the School of Science

2021-01-07

2021

Plastourgou Vasiliki

Π. Παπαζαφείρη, Αναπληρώτρια Καθηγήτρια Φυσιολογίας Ζώων, Τμήμα Βιολογίας,
ΕΚΠΑ
Σ. Ευθυμιόπουλος, Καθηγητής Φυσιολογίας Ζώων & Ανθρώπου-Νευροβιολογίας,
Τμήμα Βιολογίας, ΕΚΠΑ
Ι. Αγγελή, Επίκουρη Καθηγήτρια Φυσιολογίας Ζώων, Τμήμα Βιολογίας, ΕΚΠΑ

Επίδραση της βιταμίνης D σε σηματοδοτικούς μηχανισμούς κατά τη νευροδιαβίβαση και τη γήρανση

Greek
English

Effect of vitamin D on signaling mechanisms during neurotransmission and aging

Aging is an inevitable phenomenon, as it occurs with increasing age. However, many
diseases such as neurodegenerative Alzheimer's disease (AD) have been associated
with it. A common factor between the burden on cells leading to the ageing phenotype
and AD seems to be the excessive increase in divalent calcium ion. In addition, another
common element between these two conditions is the deficiency of vitamin D, which
acts as a regulator of ion homeostasis inside the cell. For this purpose, experiments
were performed studying whether the vitamin can affect them and through which
signaling pathways. At the same time, the effect of conditioned medium from young
fibroblasts (CMy) on aged and nerve cells was tested. The results showed that the
vitamin could not reverse the aging phenotype, when some proteins already appeared
elevated such as APP, while not affecting signaling pathway proteins. The only effect
occurs in the APP of young cells. The results of immunoblotting were also confirmed
through the results of immunocytochemistry. In addition, the vitamin has been shown
to inhibit fibroblast migration, while CMy promotes their transport and increases the
expression of proteins associated with survival and migration. In neurodegeneration,
the steroid molecule appears to work better by activating AKT, APP and STIM, unable
to inhibit the reduction of pERKs. CMy, together with CMo used as a simulation form,
increases the proteins under study and this increase remains 48 hours after the effect
while still acting. In neurodegeneration, the effects of immunoblotting were also verified
by immunocytochemistry. Finally, the evacuation of cells from calcium and their
counting, showed that vitamin and CMy provide an improved picture of cells before
they undergo some degeneration.

Science

Neurodegenerative, aging, vitamin D, CMy. calcium

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https://pergamos.lib.uoa.gr/uoa/dl/object/2932089