Κατεύθυνση Απομόνωση, Ανάπτυξη, Παραγωγή και Έλεγχος Βιοδραστικών Φυσικών ΠροϊόντωνLibrary of the School of Science
Μαρία Χαλαμπαλάκη, Επίκουρη Καθηγήτρια, Τμήμα Φαρμακευτικής, Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών
Αλέξιος-Λέανδρος Σκαλτσούνης, Καθηγητής, Τμήμα Φαρμακευτικής, Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών
Σοφία Μητάκου, Καθηγήτρια, Τμήμα Φαρμακευτικής, Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών
Φυτοχημική μελέτη των φύλλων της μαστίχας Χίου (Pistacia lentiscus L.-Anacardiaceae)-Μεταβολομική μελέτη
Phytochemical study of the leaves of Chios mastic (Pistacia lentiscus L.-Anacardiaceae) -Metabolomic analysis
The mastic tree, Pistacia lentiscus L. of the family Anacardiaceae, grows in the southern part of the island of Chios, in the well-known "Mastihohória". Despite its long history and its ethnopharmacological significance, there is a limited number of phytochemical studies about the leaves of the mastic tree and even more limited are the studies on the biological properties of their secondary metabolites.
The aim of the thesis moved on two parallel axes. The first goal of the study was the isolation and the identification of the secondary metabolites in the leaves of the mastic tree, as well as the evaluation of the antifungal activity of both total extracts and specific isolated pure compounds that will result from the experimental procedure.
The second goal was the development and application of modern approaches using analytical techniques, such as Nuclear Magnetic Resonance-NMR and Liquid Chromatography connected to a High Resolution Mass Spectrometer-LC-HRMS for the recording of the metabolic profile of the extracts that come from the leaves of the mastic tree. More specifically, a non-targeted metabolomic study (NMR-based metabolic profiling) was attempted, in order to highlight the alterations and potential biomarkers between different collections from different geographical origins, collection periods and leaf and mastic tree ages. In addition, the dereplication method (identification of known molecules in complex mixtures) was used in an attempt to characterise the metabolome of the leaves by using the LC-HRMS technique. No available data of such combinatorial studies have been reported to date in any of the species of the genus Pistacia.
Finally, an additional goal which facilitate the identification of biomarkers was the application of statistical tools in NMR data and in particular the STOCSY (Statistical Total Correlation Spectroscopy) tool.
Main subject category:
Other subject categories:
Pistacia lentiscus, isolation, leaves, metabolomics, mastic tree, metabolites, biomarkers
Number of references:
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