Unit:
Κατεύθυνση Κοσμητολογία-ΔερματοφαρμακολογίαLibrary of the School of Science
Author:
Ladopoulos Georgios
Supervisors info:
Σκαλτσά Ελένη, Καθηγήτρια, Τμήμα Φαρμακευτικής, Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών
Αγγελική Κουρουνάκη
Καθηγήτρια, Τομέας Φαρμακευτικής Χημείας, Τμήμα Φαρμακευτικής, Σχολή Επιστημών Υγείας,
Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών.
Μιχαήλ Ράλλης
Αναπληρωτής Καθηγητής, Τομέας Φαρμακευτικής Τεχνολογίας, Τμήμα Φαρμακευτικής, Σχολή
Επιστημών Υγείας, Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών.
Original Title:
Dioscorea communis (L.) Caddick & Wilkin (Dioscoreaceae): Φαρμακογνωστική μελέτη & Ανάπτυξη ζωικού προτύπου ακμής για τον έλεγχο θεραπευτικών παραγόντων
Translated title:
Dioscorea communis (L.) Caddick & Wilkin (Dioscoreaceae): Α pharmacognostic approach & Development of a murine acne model for screening therapeutic agents.
Summary:
Acne vulgaris is a chronic, inflammatory disease with a high prevalence worldwide, that affects the pilosebaceous unit and manifests with various clinical symptoms. Acne pathogenesis is multifactorial and to this date has not been fully elucidated. Conventional treatment options do not adequately target multiple pathogenetic mechanisms and are accompanied by adverse effects that reduce compliance. Natural products are therefore increasingly being researched for new drug development. Natural products with antibacterial, anti-inflammatory and antioxidant activities have shown promising results for the treatment of acne vulgaris. Most studies, however, are of limited translational value since they are primarily based on incomplete preclinical models. Thus, acne models that simulate human disease are essential. Extracts of Dioscorea communis (L.) Caddick & Wilkin [syn. Tamus communis (L.)] (Dioscoreaceae) have been found to exhibit mainly anti-inflammatory and antioxidant effects, which are in accordance with their chemical composition. Such effects could be useful for the treatment of acne since they target multiple pathogenetic factors of the disease. The aim of this study was to develop a reproducible and reliable animal model of acne, to be subsequently used for screening therapeutic agents and specifically D. communis extracts selected according to their chemical compositions. A murine acne model involving intradermal injection of a Cutibacterium acnes bacterial suspension was chosen. C. acnes is implicated in acne pathogenesis and this model combines multiple pathogenetic mechanisms. A comparative study of three hairless mice species - SKH-hr1, SKH-hr2 (brown) and SKH-hr2+ApoE (hybrid species with increased cholesterol levels) - was performed for the first time, to determine which species better simulates human acne. The model was evaluated and characterized by photodocumentation, biomechanics, blood work and histopathology. While all three species presented acne-like lesions, SKH-hr1 mice were deemed the most appropriate. Extracts of D. communis leaves and fruits were prepared and analyzed via chromatographic and spectroscopic methods. GC/MS analysis of the cyclohexane extract of the leaves revealed mainly hydrocarbons. Εthanolic extracts of the leaves were found to contain mainly triterpene saponins and phenolic derivatives of apigenin and kaempferol, as determined by 1H NMR and literature review.
Main subject category:
Science
Other subject categories:
Health Sciences
Keywords:
Dioscorea, Tamus, acne, mice, SKH-hr1, bacteria, Propionibacterium, natural, extract, anti-inflammatory, antioxidant
Number of references:
226
