Unit:
Κατεύθυνση Απομόνωση, Ανάπτυξη, Παραγωγή και Έλεγχος Βιοδραστικών Φυσικών ΠροϊόντωνLibrary of the School of Science
Author:
Christodoulou Eleni-Anna
Supervisors info:
Βασίλειος Ρούσσης
Καθηγητής, Τμήμα Φαρμακευτικής, Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών
Ευσταθία Ιωάννου
Επίκ. Καθηγήτρια, Τμήμα Φαρμακευτικής, Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών
Σπύρος Ε. Ζωγράφος
Ερευνητής Α’, Ινστιτούτο Χημικής Βιολογίας, Εθνικό Ίδρυμα Ερευνών
Original Title:
Μελέτη πτητικών μεταβολιτών από θαλάσσιους οργανισμούς: μελέτες πρόσδεσης σε οσφρητικές πρωτεΐνες (OBPs) του κουνουπιού Anopheles gambiae
Translated title:
Investigation of volatile metabolites from marine organisms: studies on their binding to odorant proteins (OBPs) of the mosquito Anopheles gambiae
Summary:
In the framework of the present MSc thesis the binding ability of volatile metabolites isolated from marine organisms was studied in two chemoreceptor-targets (OBPs) of the mosquito Anopheles gambiae, aiming at the discovery of compounds with potential insect repellent ability. The metabolites under study included mainly sesquiterpenes and diketopiperazines. The dissociation constant of the compounds with the odorant binding protein AgamOBP1 was determined with fluorescence assays. All investigated metabolites exhibited binding properties with AgamOBP1. In addition, the binding of selected compounds to AgamOBP5 was evaluated fluorometrically. Finally, protein crystals were developed through crystallization experiments and a preliminary binding study using 1Η STD NMR experiments was initiated, which will allow the structural study of the complexes of the compounds under investigation.
Main subject category:
Science
Other subject categories:
Health Sciences
Keywords:
Volatile metabolites; marine organisms; odorant binding proteins; mosquito; Anopheles gambiae
