Unit:
Faculty of MedicineLibrary of the School of Health Sciences
Author:
Kokkalas Nikolaos-Stilianos
Dissertation committee:
Δοντά Ισμήνη, Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
Παπαχρήστου Διονύσιος, Καθηγητής, Ιατρική Σχολή, Πανεπιστήμιο Πατρών
Κοκότης Παναγιώτης, Αναπληρωτής Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Χρονόπουλος Ευστάθιος, Αναπληρωτής Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Νικολάου Βασίλειος, Αναπληρωτής Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Βλάμης Ιωάννης Επίκουρος Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Γιαννακόπουλος Χρήστος, Επίκουρος Καθηγητής ΤΕΦΑΑ ΕΚΠΑ
Original Title:
Η επίδραση των μεσεγχυματικών κυττάρων και του πλάσματος πλούσιου σε αιμοπετάλια στη συρραφή του ισχιακού νεύρου σε επιμύες
Translated title:
The effect of mesenchymal stem cells and of platelet-rich plasma in repair of transected sciatic nerves in rats
Summary:
Introduction: Platelet-rich plasma (PRP) products and mesenchymal stem cells (MSCs) seem to have a significant potential as neurogenic therapeutic modulator systems.
Aim: This study aimed to investigate such biological blood derivatives that could enhance nerve regeneration when applied locally in the primary repair of peripheral nerve transection of an experimental rat model.
Methods: A total of 42 two-month-old male Wistar rats were divided into three “treatment” groups (control, PRP, and MSCs). All the subjects were operated under anesthesia, and the surgical site was infiltrated with either normal saline, PRP derived from the animal’s peripheral blood, or MSCs derived from the animal’s femoral bone marrow. All three groups were also sub-divided into two sub-groups based on the post-operative administration of Non-steroidal anti-inflammatory drugs (NSAIDs) or not in order to evaluate the effect of NSAIDs on the final outcome. Three months post-surgery, electromyography evaluation of both hind limbs (right operated and left non-operated) was performed. The animals were euthanized, and nerve repair specimens were prepared for histology.
Results: PRP group had a significant effect (p<0.05) on the sciatic nerve repair when compared with the control group, whereas the MSCs group had a positive effect but was not statistically significant (p=0.2). The number of counted neural axons at the area distal to the nerve repair site were significantly repetitive (p<0.05) in both the PRP and MSCs groups when compared with the control group.
Conclusions: Both PRP and MSCs appear to play an essential role in the enhancement of nerve repair in terms of functionality and histology. MSCs group demonstrated a positive effect, whereas the PRP group showed statistically significant better results.
Main subject category:
Health Sciences
Keywords:
Platelet rich plasma, Mesenchymal stem cells, Nerve repair, Nerve regeneration
Number of references:
161
