Electrochemical detection of glucose in artificial sweat using sensors modified with complexes

Postgraduate Thesis uoadl:2963048 29 Read counter

Κατεύθυνση Αναλυτική Χημεία-Διασφάλιση ποιότητας
Library of the School of Science
Deposit date:
Vasileiou Foteini
Supervisors info:
Χρήστος Κόκκινος, Επίκουρος Καθηγητής, Τμήμα Χημείας, ΕΚΠΑ
Original Title:
Ηλεκτροχημικός προσδιορισμός γλυκόζης σε τεχνητό ιδρώτα με χρήση αισθητήρων τροποποιημένων με σύμπλοκες ενώσεις
Translated title:
Electrochemical detection of glucose in artificial sweat using sensors modified with complexes
For the diagnosis and self-monitoring of diabetes, the detection of blood glucose is a commonly used method. Despite the excellent selectivity and sensitivity of glucose enzymatic electrochemical sensors, which are prepared using the enzyme glucose oxidase (GOx) in order to catalyze glucose oxidation, the use of enzymes includes many disadvantages, such as the high cost of enzymes and their inadequate stability due to their dependence on environmental conditions (temperature, pH and humidity). In contrast, the non-enzymatic glucose sensor operates with a more attractive method which is based on the direct electrocatalysis of glucose on the electrode surface. These non-enzymatic sensors have significant advantages such as low cost, longer life and stability and fast performance – response of the analysis. On the other hand, non-invasive glucose testing is a better route towards easy and painless glucose monitoring, as sweat contains a plethora of biomarkers of essential relevance to the assessment of physiological health. In addition, the working electrode material is a critical factor in determining the electrocatalytic efficiency. In the present research work, a new type of electrochemical sensors was developed for the detection of glucose levels in artificial sweat. Three copper complexes and two oxides of copper were tested as modifiers of carbon paste electrodes for the non-enzymatic glucose sensing and the {[Cu (PhCOO) (H2O)] x (PhCOO) (H2O)}n presented the best electroanalytical response towards glucose monitoring. Different parameters which affect the response of the sensor were tested and optimized (modifier content 1% w/w, pre-concentration time and potential: 360s and -1.4 V). Under the selected conditions the determination of glucose in artificial sweat offered a limit of detection (LOD) of 5 μM without interference from common electroactive metabolites. It is worth mentioning that a very good response of the working electrode was also observed for uric acid determination and thus the modified electrode was also applied to the voltametric determination of uric acid in artificial sweat, with LOD = 4,6 μM.
Main subject category:
glucose, diabetes, artificial sweat, complex, copper (Cu), uric acid
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