Κατεύθυνση Βιομηχανική Φαρμακευτική
Library of the School of Science

2023-05-16

2023

Manolopoulou Vasiliki

Κωνσταντίνος Δεμέτζος, Καθηγητής Τμήμα Φαρμακευτικής ΕΚΠΑ,
Μαριλένα Βλάχου-Κωνσταντινίδου, Αναπληρώτρια Καθηγήτρια Τμήμα Φαρμακευτικής ΕΚΠΑ,
Νατάσσα Πίππα, Επίκουρη Καθηγήτρια Τμήμα Φαρμακευτικής ΕΚΠΑ

Ανάπτυξη λιπιδικών νανοσωματιδίων υγρών κρυστάλλων με μη ιοντικές επιφανειοδραστικές ουσίες για μεταφορά της ένωσης κερκετίνης

Greek

Development of liquid crystalline lipid nanoparticles with non-ionic surfactants for quercetin delivery

Nanotechnology is widely used for the diagnosis and treatment of many diseases. Lyotropic liquid crystals are classified, as drug delivery nanosystems due to their unique morphological characteristics. Thus, liquid crystals can encapsulate both hydrophilic and hydrophobic bioactive molecules, improve the pharmacokinetics of the encapsulated drug, increase its effectiveness and reduce possible toxic effects. The present study focuses on the development, physicochemical characterization and the in vitro biological evaluation of both blank and quercetin-loaded phytantriol (PHYT) liquid crystalline nanoparticles mixed with the Poloxamer 407, which acts as stabilizer, as well as with different nonionic surfactant molecules (Tween 80 and/or Span 80). The liquid crystalline systems, both blank and quercetin-loaded, were prepared by the Top-Down (TD) method. In addition, the physicochemical properties of the prepared nanosystems, such as size and size distribution, were evaluated by the Dynamic Light Scattering (DLS) technique over time, while their ζ-potential was evaluated with the Electrophoretic Light Scattering (ELS) technique. The physicochemical characteristics were influenced by formulation parameters such as the surfactant type. Furthermore, the entrapment efficiency (EE) of quercetin was more than 80% in all prepared systems. Moreover, the effect of the serum proteins presence on the physicochemical behavior of the nanosystems was evaluated. Last, the in vitro biological evaluation was performed by using the MTT assay, to evaluate the cytotoxicity of the nanosystems on the WM164 melanoma cell line as well as the Caco-2 cancer cell line. In both cases, the nanosystems were found to exhibit good biocompatibility.

Science

liquid crystalline lipid nanoparticles, lipid nanoparticles, drug delivery nanosystems, quercetin, phytantriol, non-ionic surfactants

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File access is restricted until 2026-05-26.

https://pergamos.lib.uoa.gr/uoa/dl/object/3328374