Τίτλος:
Frequency, clinical characteristics, and genotype distribution of rotavirus gastroenteritis in Greece (2007-2008)
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Rotavirus is the leading cause of acute gastroenteritis among young children worldwide. A prospective multi-center study was conducted (2007-2008) in five Pediatric Hospitals to determine the prevalence, the clinical characteristics, and genotype distribution of rotavirus infection in Greece. Faecal samples were examined for the presence of group A rotavirus antigen by immunochromatography. Rotavirus strains were subjected to G and P genotyping by reverse-transcriptase polymerase chain reaction (PCR) and sequencing. A total of 393 children (216 boys) of median age 23 months, participated in the study. Rotavirus was the cause of acute gastroenteritis in 166 children, 42.3% (CI 95%, 37.4-47.1%) of non-hospitalized and 47.8% (CI 95%, 41.7-53.9%) of hospitalized patients. Rotavirus gastroenteritis occurred between December and April in 78.6% of the cases. Most children with RVG (77.8%) were between 3 months and 3 years old. The mean value of Clark severity score was 12.9 ± 5.1 for RVG and 10.5 ± 4.9 for non-RVG (P < 0.01). Genotypes were determined in 117 strains and their distribution was as following: G1P[8], 49%; G2P[4], 31%; G4P[8], 10%; G9P[8], 9%; and G8P[14], 1%. In conclusion, rotavirus is a frequent cause of acute gastroenteritis in Greece. The genotypes circulating are similar with those of other European countries. Copyright © 2010 Wiley-Liss, Inc.
Συγγραφείς:
Koukou, D.
Grivea, I.
Roma, E.
Tsioni, H.
Trimis, G.
Galanakis, E.
Farmaki, E.
Iosifidis, E.
Michos, A.
Siamopoulou-Mavridou, A.
Kalmanti, M.
Papadopoulou, H.
Roilides, E.
Theodoridou, M.
Syrogiannopoulos, G.A.
Syriopoulou, V.
Περιοδικό:
Journal of Medical Virology
Λέξεις-κλειδιά:
virus antigen, acute gastroenteritis; article; child; chromatography; clinical feature; clinical trial; controlled clinical trial; controlled study; disease severity; feces; feces analysis; female; gastroenteritis; gene sequence; genotype; Greece; human; infant; major clinical study; male; multicenter study; nonhuman; pediatric hospital; preschool child; prevalence; prospective study; reverse transcription polymerase chain reaction; Rotavirus; species distribution, Antigens, Viral; Child, Preschool; Feces; Female; Gastroenteritis; Genotype; Greece; Humans; Infant; Male; Prevalence; Prospective Studies; Reverse Transcriptase Polymerase Chain Reaction; RNA, Viral; Rotavirus; Rotavirus Infections; Sequence Analysis, DNA, Rotavirus; Rotavirus A
