Περίληψη:
Aspergillus species originating from Greece were examined by morphological and molecular criteria to explore the diversity of this genus. The phylogenetic relationships of these species were determined using sequences from the ITS and IGS region of the nuclear rRNA gene complex, two nuclear genes (β-tubulin (benA) and RNA polymerase II second largest subunit (rpb2)) and two mitochondrial genes (small rRNA subunit (rns) and cytochrome oxidase subunit I (cox1)) and, where available, related sequences from databases. The morphological characters of the anamorphs and teleomorphs, and the single gene phylogenetic trees, differentiated and placed the species examined in the well-supported sections of Aenei, Aspergillus, Bispori, Candidi, Circumdati, Clavati, Cremei, Flavi, Flavipedes, Fumigati, Nidulantes, Nigri, Restricti, Terrei, Usti, and Zonati, with few uncertainties. The combined use of the three commonly employed nuclear genes (benA, rpb2, and ITS), the IGS region, and two less often used mitochondrial gene sequences (rns and cox1) as a single unit resolved several taxonomic ambiguities. A phylogenetic tree was inferred using Neighbour-Joining, Maximum Parsimony, and Bayesian methods. The strains examined formed seven well-supported clades within the genus Aspergillus. Altogether, the concatenated nuclear and mitochondrial sequences offer additional tools for an improved understanding of phylogenetic relationships within this genus. © 2013 Antonios Krimitzas et al.
Συγγραφείς:
Krimitzas, A.
Pyrri, I.
Kouvelis, V.N.
Kapsanaki-Gotsi, E.
Typas, M.A.
Λέξεις-κλειδιά:
beta tubulin; cytochrome c oxidase; nuclear RNA; ribosome RNA; RNA polymerase II; fungal DNA, Aenei; anamorph; article; Ascomycetes; Aspergillus; Aspergillus aculeatus; Aspergillus aeneus; Aspergillus arenarius; Aspergillus awamori; Aspergillus bisporus; Aspergillus brunneouniseriatus; Aspergillus campestris; Aspergillus clavatoflavus; Aspergillus clavatus; Aspergillus elongatus; Aspergillus flavus; Aspergillus fumigatus; Aspergillus giganteus; Aspergillus janus; Aspergillus niger; Aspergillus niveus; Aspergillus ochraceus; Aspergillus oryzae; Aspergillus parasiticus; Aspergillus puniceus; Aspergillus restrictus; Aspergillus sclerotiorum; Aspergillus sydowii; Aspergillus terreus; Aspergillus ustus; Aspergillus versicolor; Australia; bacterial gene; Bayesian learning; Bispori; Candidi; cell nucleus; Circumdati; Clavati; Cremei; emericella nidulans; Emericella variecolor; Eurotium amstelodami; Eurotium rubrum; Flavi; Flavipedes; Fumigati; gene sequence; Greece; India; Metarhizium anisopliae; Mexico; mitochondrial gene; Neosartorya fischeri; Nidulantes; Nigri; nonhuman; nucleotide sequence; Panama; parsimony analysis; phylogeny; Restricti; Somalia; species diversity; teleomorph; Terrei; United Kingdom; United States; Usti; Zonati; cell nucleus; classification; cytology; fungal gene; genetics; isolation and purification; mitochondrial gene; phenotype; phylogeny; Aspergillus; fungal gene; isolation and purification, Aspergillus, Aspergillus; Base Sequence; Cell Nucleus; DNA, Fungal; Genes, Fungal; Genes, Mitochondrial; Greece; Phenotype; Phylogeny, Aspergillus; Base Sequence; Cell Nucleus; DNA, Fungal; Genes, Fungal; Genes, Mitochondrial; Greece; Phenotype; Phylogeny
