Τίτλος:
Prevalence of a plasmid-mediated type II dihydrofolate reductase gene among trimethoprim-resistant urinary pathogens in greek hospitals
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
The genetic basis of trimethoprim resistance was examined in 24 Klebsiella pneumoniae, 27 Enterobacter cloacae, five Enterobacter aerogenes and nine Serratia marcescens urinary isolates from five hospitals in Greece. Analysis of the 65 isolates by serotyping and phage-typing identified 53 distinct strains. Thirty-eight isolates (15 K. pneumoniae, 19 E. cloacae, two E. aerogenes and two S. marcescens) hybridized with a probe specific for a gene encoding type II dihydrofolate reductase (DHFR). Three of the K. pneumoniae and four of the E. cloacae isolates which reacted with this probe also hybridized with probes specific for type I DHFR and transposon Tn7. Two E. cloacae isolates hybridized only with the probe for type I DHFR, while a further three isolates hybridized only with the type I DHFR and Tn7 probes. None of the isolates hybridized with a probe for type V DHFR. The plasmids in transconjugants derived from 40 isolates were analysed by digestion with restriction enzymes and Southern blotting. Eighteen (45%) of the donors (12 K. pneumoniae and 6 E. cloacae) produced transconjugants containing plasmids of about 95 kb in size, while transconjugants from the other donors had plasmids in the range 100-185 kb. Of the 18 transconjugants containing a 95 kb plasmid, 15 had similar restriction endonuclease digest patterns, although they varied in terms of the range of antimicrobial resistances which they encoded. When EcoRI digests of these 15 plasmids were hybridized with the type II DHFR probe, a 23 kb common band reacted with the probe. These findings indicate that the high level of trimethoprim resistance in these genera resulted, in part, from the spread of related plasmids encoding production of type II DHFR. © 1992 by The British Society for Antimicrobial Chemotherapy.
Συγγραφείς:
Tsakris, A.
Vatopoulos, A.C.
Johnson, A.P.
Pitt, T.L.
Legakis, N.J.
Tzouvelekis, L.S.
Περιοδικό:
The Journal of antimicrobial chemotherapy
Λέξεις-κλειδιά:
amikacin; ampicillin; dihydrofolate reductase; netilmicin; sulfamethoxazole; tetracycline; tobramycin; trimethoprim, antibiotic resistance; article; bacteriophage typing; bacterium conjugation; bacterium isolation; enterobacter aerogenes; enterobacter cloacae; gene; greece; hospital; klebsiella pneumoniae; minimum inhibitory concentration; nonhuman; prevalence; priority journal; serotyping; serratia marcescens; southern blotting; transposon; urinary tract infection, Cross Infection; DNA Probes; Drug Resistance, Microbial; Enterobacter; Genes, Bacterial; Greece; Klebsiella pneumoniae; Microbial Sensitivity Tests; Prevalence; R Factors; Serotyping; Serratia marcescens; Tetrahydrofolate Dehydrogenase; Trimethoprim; Urinary Tract Infections
DOI:
10.1093/jac/29.4.405
