Τίτλος:
Human L-Dopa decarboxylase interaction with annexin V and expression during apoptosis
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
L-Dopa Decarboxylase (DDC) is a pyridoxal requiring enzyme that catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (L-Dopa) to Dopamine (DA). The function of DDC in physiological and pathological biochemical pathways remains poorly understood, while the function and regulation of human DDC isoforms is almost completely elusive. We have shown that Annexin V, a fundamental apoptosis marker, is an inhibitor of L-Dopa decarboxylase activity. Here we show the interaction of both the full-length DDC and the truncated isoform alternative DDC (Alt-DDC) with Annexin V in human tissue and cell lines. Interestingly, DDC isoform expression is enhanced or remains unaffected following staurosporine (STS) treatment, despite increased levels of cytotoxicity and apoptosis. The findings presented here provide novel insights concerning the involvement of DDC in programmed cell death. © 2020 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM)
Συγγραφείς:
Chalatsa, I.
Arvanitis, N.
Arvanitis, D.
Tsakou, A.C.
Kalantzis, E.D.
Vassiliou, A.G.
Sideris, D.C.
Frakolaki, E.
Vassilaki, N.
Vassilacopoulou, D.
Λέξεις-κλειδιά:
aromatic levo amino acid decarboxylase; lipocortin 5; staurosporine; aromatic levo amino acid decarboxylase; cobalt; cobalt chloride; DDC protein, human; enzyme inhibitor; isoprotein; lipocortin 5; staurosporine, animal cell; apoptosis; Article; CHO cell line; controlled study; cytotoxicity; female; HEK293 cell line; HeLa cell line; Huh-7 cell line; human; human cell; human tissue; immunoprecipitation; molecular weight; nonhuman; polyacrylamide gel electrophoresis; protein protein interaction; real time reverse transcription polymerase chain reaction; SH-SY5Y cell line; upregulation; animal; apoptosis; cell death; cell line; drug effect; genetics; hamster; metabolism; placenta; pregnancy; tumor cell line, Animals; Annexin A5; Apoptosis; Aromatic-L-Amino-Acid Decarboxylases; Cell Death; Cell Line; Cell Line, Tumor; Cobalt; Cricetinae; Enzyme Inhibitors; Female; Humans; Placenta; Pregnancy; Protein Isoforms; Staurosporine
DOI:
10.1016/j.biochi.2020.08.010
