Τίτλος:
Effect of nanostructured TiO2 crystal phase on photoinduced apoptosis of breast cancer epithelial cells
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Purpose: The use of nanoparticles has seen exponential growth in the area of health care, due to the unique physicochemical properties of nanomaterials that make them desirable for medical applications. The aim of this study was to examine the effects of crystal phase-nanostructured titanium dioxide particles on bioactivity/cytotoxicity in breast cancer epithelial cells. Materials and methods: Cultured Michigan Cancer Foundation (MCF)-7 and human breast adenocarcinoma (MDA-MB-468) breast cancer epithelial cells were exposed to ultraviolet A light (wavelength 350 nm) for 20 minutes in the presence of aqueous dispersions of two different nanostructured titanium dioxide (TiO2) crystal phases: anatase and an anatase-rutile mixture. Detailed characterization of each titanium dispersion was performed by dynamic light scattering. A 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) colorimetric assay was employed to estimate the percentage of viable cells after each treatment. Western blot analysis of protein expression and characterization, as well as a deoxyribonucleic acid (DNA)-laddering assay, were used to detect cell apoptosis. Results: Our results documented that 100% anatase TiO2 nanoparticles (110-130 nm) exhibited significantly higher cytotoxicity in the highly malignant MDA-MB-468 cancer cells than anatase-rutile mixtures (75%/25%) with the same size. On the contrary, MCF-7 cells (characterized by low invasive properties) were not considerably affected. Exposure of MDA-MB-468 cells to pure anatase nanoparticles or anatase-rutile mixtures for 48 hours resulted in increased proapoptotic Bax expression, caspase-mediated poly(adenosine diphosphate ribose) polymerase (PARP) cleavage, DNA fragmentation, and programmed cell death/apoptosis. Conclusion: The obtained results indicated that pure anatase TiO2 nanoparticles exhibit superior cytotoxic effects compared to anatase-rutile mixtures of the same size. The molecular mechanism of TiO2 nanoparticle cytotoxicity involved increased Bax expression and caspase-mediated PARP inactivation, thus resulting in DNA fragmentation and cell apoptosis. © 2014 Lagopati et al.
Συγγραφείς:
Lagopati, N.
Tsilibary, E.-P.
Falaras, P.
Papazafiri, P.
Pavlatou, E.A.
Kotsopoulou, E.
Kitsiou, P.
Περιοδικό:
International Journal of Nanomedicine
Εκδότης:
Dove Medical Press Ltd
Λέξεις-κλειδιά:
3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide; caspase; DNA; nanoparticle; protein Bax; protein bcl 2; titanium dioxide; antineoplastic agent; nanomaterial; protein bcl 2; titanium; titanium dioxide, apoptosis; article; breast adenocarcinoma; cancer cell destruction; cell viability; colorimetry; controlled study; crystal structure; DNA fragmentation; DNA fragmentation assay; drug cytotoxicity; drug mechanism; epithelium cell; human; human cell; light scattering; MCF 7 cell line; particle size; protein cleavage; protein expression; tumor invasion; ultraviolet A radiation; Western blotting; apoptosis; breast tumor; cell survival; chemistry; drug effects; female; gene expression regulation; metabolism; radiation response; ultraviolet radiation, Antineoplastic Agents; Apoptosis; Breast Neoplasms; Cell Survival; DNA Fragmentation; Epithelial Cells; Female; Gene Expression Regulation, Neoplastic; Humans; MCF-7 Cells; Nanostructures; Proto-Oncogene Proteins c-bcl-2; Titanium; Ultraviolet Rays
