Τίτλος:
Application of nano-hydroxyapatite/chitosan scaffolds on rat calvarial critical-sized defects: A pilot study
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background: The purpose of this pilot study was to evaluate for the first time the effect of 75/25 w/w nano-Hydroxyapatite/Chitosan (nHAp/CS) scaffolds on Guided Bone Regeneration (GBR) in rat calvarial critical-sized defects (CSDs). Material and Methods: Six adult Sprague Dawley rats, 3 males and 3 females, were used. Two CSDs, full thickness and 5mm in diameter, were trephined in both sides of the parietal bone. The right CSD was filled with nHAp/ CS scaffold, while the left CSD remained empty, as the control group. The wound was sutured in layers. Rats were euthanized with diethyl ether inhalation at 2, 4 and 8 weeks after surgical procedure. Histological and histomorphometric analysis was performed within distinct regions of interest (ROI): the lateral area inward of the middle sagittal seam; the lateral area outward of the middle sagittal seam and the central area. Results: The mean surface of newly formed bone (in μm 2 ) in the lateral area inward of the middle sagittal seam of all rats was significantly higher (P=0.039) in the experimental group (91733.00±38855.60) than the control group (46762.17±25507.97). The NOex-c, defined as total number of osteocytes (OST) in newly formed bone surface in experimental group [experimental OST] minus the total number of osteocytes in newly formed bone surface in control group [control OST], was significantly greater (P=0.029) at 4 th week post-surgery. Within the experimental group, a statistically significant increase (P=0.042) in the surface of newly formed bone was noticed in rats euthanized in 4 th week compared with rats euthanized in 2 nd week after surgery in the lateral area inward of the middle sagittal seam. Conclusions: The results of this study suggest that 75/25 w/w nHAp/CS scaffolds should be considered as a suitable biomaterial for GBR. © Medicina Oral S. L. C.I.F.
Συγγραφείς:
Chatzipetros, E.
Christopoulos, P.
Donta, C.
Tosios, K.I.
Tsi-Ambas, E.
Tsiourvas, D.
Kalogirou, E.-M.
Tsiklakis, K.
Περιοδικό:
Medicine Oral: Patologia Oral y Cirugia Bucal
Εκδότης:
Medicina Oral S.L.
Λέξεις-κλειδιά:
caprofen; carprofen; chitosan; enrofloxacin; hydroxyapatite; molecular scaffold; polyglactin; sodium chloride; unclassified drug; biomaterial; chitosan; hydroxyapatite; nanoparticle, adult; analysis of variance; anatomy; animal experiment; animal model; animal tissue; Article; biological activity; bone regeneration; calvaria; cell infiltration; controlled study; deacetylation; elasticity; female; freeze drying; histology; in vitro study; inflammation; laminar flow; male; microscopy; molecular mechanics; molecular weight; morphometry; nonhuman; observational study; osteocyte; parietal bone; pathology; rat; research; animal; bone development; drug effect; pilot study; skull; Sprague Dawley rat; surgery; tissue scaffold, Animals; Biocompatible Materials; Bone Development; Chitosan; Female; Hydroxyapatites; Male; Nanoparticles; Pilot Projects; Rats; Rats, Sprague-Dawley; Skull; Tissue Scaffolds
DOI:
10.4317/medoral.22455
