Τίτλος:
SARS-CoV-2 antigenemia as a confounding factor in immunodiagnostic assays: A case study
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Humoral immunity has emerged as a vital immune component against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Nevertheless, a subset of recovered Coronavirus Disease-2019 (COVID-19) paucisymptomatic/asymptomatic individuals do not generate an antibody response, constituting a paradox. We assumed that immunodiagnostic assays may operate under a competitive format within the context of antigenemia, potentially explaining this phenomenon. We present a case where persistent antigenemia/viremia was documented for at least 73 days post-symptom onset using ‘in-house’ methodology, and as it progressively declined, seroconversion took place late, around day 55, supporting our hypothesis. Thus, prolonged SARS-CoV-2 anti-genemia/viremia could mask humoral responses, rendering, in certain cases, the phenomenon of ‘non-responders’ a misnomer. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Συγγραφείς:
Belogiannis, K.
Florou, V.A.
Fragkou, P.C.
Ferous, S.
Chatzis, L.
Polyzou, A.
Lagopati, N.
Vassilakos, D.
Kittas, C.
Tzioufas, A.G.
Tsiodras, S.
Sourvinos, G.
Gorgoulis, V.G.
Περιοδικό:
Virus Bulletin
Λέξεις-κλειδιά:
antipyretic agent; hematoxylin; metformin; monoclonal antibody; virus antigen; immunoglobulin G; virus antibody; virus antigen, adult; antibody detection; antibody production; antibody response; antigen blood level; Article; bioinformatics; case report; clinical article; cohort analysis; confounding variable; controlled study; coronavirus disease 2019; cycle threshold value; enzyme linked immunosorbent assay; fever; high throughput sequencing; human; human tissue; humoral immunity; immunohistochemistry; male; nasopharyngeal swab; reverse transcription polymerase chain reaction; RNA extraction; sandwich ELISA; sensitivity and specificity; seroconversion; serodiagnosis; Severe acute respiratory syndrome coronavirus 2; viremia; virus load; young adult; antibody combining site; blood; diagnosis; immunology; metabolism; virology, Antibodies, Viral; Antigens, Viral; Binding Sites, Antibody; COVID-19; COVID-19 Serological Testing; Humans; Immunity, Humoral; Immunoglobulin G; Male; SARS-CoV-2; Sensitivity and Specificity; Seroconversion; Young Adult
