Τίτλος:
Role of Cathepsin S in Periodontal Inflammation and Infection
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Cathepsin S is a cysteine protease and regulator of autophagy with possible involvement in periodontitis. The objective of this study was to investigate whether cathepsin S is involved in the pathogenesis of periodontal diseases. Human periodontal fibroblasts were cultured under inflammatory and infectious conditions elicited by interleukin-1β and Fusobacterium nucleatum, respectively. An array-based approach was used to analyze differential expression of autophagy-associated genes. Cathepsin S was upregulated most strongly and thus further studied in vitro at gene and protein levels. In vivo, gingival tissue biopsies from rats with ligature-induced periodontitis and from periodontitis patients were also analyzed at transcriptional and protein levels. Multiple gene expression changes due to interleukin-1β and F. nucleatum were observed in vitro. Both stimulants caused a significant cathepsin S upregulation. A significantly elevated cathepsin S expression in gingival biopsies from rats with experimental periodontitis was found in vivo, as compared to that from control. Gingival biopsies from periodontitis patients showed a significantly higher cathepsin S expression than those from healthy gingiva. Our findings provide original evidence that cathepsin S is increased in periodontal cells and tissues under inflammatory and infectious conditions, suggesting a critical role of this autophagy-associated molecule in the pathogenesis of periodontitis. © 2017 S. Memmert et al.
Συγγραφείς:
Memmert, S.
Damanaki, A.
Nogueira, A.V.B.
Eick, S.
Nokhbehsaim, M.
Papadopoulou, A.K.
Till, A.
Rath, B.
Jepsen, S.
Götz, W.
Piperi, C.
Basdra, E.K.
Cirelli, J.A.
Jäger, A.
Deschner, J.
Περιοδικό:
Mediators of Inflammation
Λέξεις-κλειδιά:
autophagy related gene 3 protein; autophagy related protein; bcl2 antagonist killer 1 protein; cathepsin S; chemokine receptor CXCR4; death associated protein kinase 1; dna damage regulated autophagy modulator 1; eukaryotic translation initiation factor 4 gamma; glyceraldehyde 3 phosphate dehydrogenase; hypoxanthine phosphoribosyltransferase; immunity related gtpase family m; insulin; interleukin 1beta; messenger RNA; nuclear factor of kappa light polypeptide gene enhancer in b cell 1; peptides and proteins; phosphoinositide 3 kinase catalytic gamma polypeptide; protein; protein bcl 2; protein Bid; protein kinase; somatomedin C; TATA binding protein; transmembrane protein 74; tumor necrosis factor related apoptosis inducing ligand; unclassified drug; cathepsin; cathepsin S, adolescent; adult; aged; animal experiment; animal model; animal tissue; Article; autophagy; cells by body anatomy; child; clinical article; controlled study; experimental periodontitis; fibroblast; Fusobacterium nucleatum; gene expression level; gene expression profiling; gene expression regulation; genetic association; genetic transcription; gingival biopsy; human; human cell; human tissue; in vitro study; in vivo study; male; molecular pathology; mRNA expression level; nonhuman; normal human; periodontal disease; periodontal ligament; periodontal ligament cell; periodontitis; periodontium; protein expression level; rat; upregulation; animal; cell culture; enzymology; female; gingiva; metabolism; periodontitis; physiology; young adult, Adolescent; Adult; Animals; Autophagy; Cathepsins; Cells, Cultured; Child; Female; Gingiva; Humans; Male; Periodontitis; Rats; Young Adult
DOI:
10.1155/2017/4786170
