Τίτλος:
A high mobility group protein from the dipteran insects Ceratitis capitata and Bactrocera oleae
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Nuclei from Bactrocera oleae and Ceratitis capitata larvae contain a major protein that shares most of the characteristics of vertebrate high mobility group (HMG) proteins. Proteins are extracted from nuclei with 0.35 M NaCl, are soluble in 5% perchloric acid, are relatively small (molecular weight in the range of 10-16 kDa), and have both a high basic and a high acidic amino acid content. The amino acid constitution of these proteins is similar to that of the HMGB protein family of vertebrates. The proteins cross-react with antibodies raised against the HMGD chromosomal protein of Drosophila melanogaster. The possible relatedness of these proteins to high mobility group proteins is discussed.
Συγγραφείς:
Aleporou-Marinou, V.
Deli, C.
Ninios, Y.
Agelopoulou, B.
Marinou, H.
Patargias, T.
Περιοδικό:
Biochemical Genetics
Λέξεις-κλειδιά:
antibody; high mobility group protein; perchloric acid, amino acid composition; article; Bactrocera oleae; cross reaction; Drosophila melanogaster; extraction; insect; larva; Mediterranean fruit fly; molecular weight; nonhuman; protein analysis; protein family; protein isolation; vertebrate, Animals; Blotting, Western; Ceratitis capitata; Drosophila melanogaster; Electrophoresis, Polyacrylamide Gel; High Mobility Group Proteins; Histones; Nuclear Proteins; Tephritidae, Bactrocera oleae; Capitata; Ceratitis; Ceratitis capitata; Diptera; Drosophila melanogaster; Hexapoda; Insecta; Melanogaster; Vertebrata
DOI:
10.1023/A:1025558318077
