Τίτλος:
Chemometrical development and comprehensive validation of a solid phase microextraction/gas chromatography-mass spectrometry methodology for the determination of important free and bound primary aromatics in Greek wines
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
In this work, a solid-phase microextraction/gas chromatography-mass spectrometry (SPME/GC-MS) methodology was developed for the determination of 21 free and glycosidically-bound volatile varietal aroma compounds in wines. Initially, a comparison was made of 5 commercially available SPME fibers for the isolation/preconcentration of the target compounds in the headspace (HS) and direct immersion (DI) modes. The statistical significance of the microextraction variables was evaluated using a 2-level Plackett-Burman experimental design; the most significant variables were further optimized using a modified Simplex procedure. Using the selected conditions, a GC-MS method was fully validated for the quantitative determination of the 21 free primary aroma compounds. The hydrophilic bound precursors were isolated by solid-phase extraction (SPE), enzymatically hydrolyzed to liberate them as the free compounds and further detected by SPME/GC-MS. The method has been successfully applied to the analysis of 20 Greek white wine samples. © 2013 Elsevier B.V.
Συγγραφείς:
Metafa, M.
Economou, A.
Περιοδικό:
Journal of Chromatography A
Λέξεις-κλειδιά:
Chemometrics; Gas chromatography-mass spectrometry; Solid-phase microextraction; Validation; Volatile aroma compounds, Chemistry; Chromatography; Wine, Gas chromatography, 2 octanol; 3 hexenol; alcohol; alpha ionone; aromatic compound; benzyl alcohol; beta citral; beta damascenone; beta ionone; cis furan linalool oxide; cis rose oxide; citral; citronellol; dimeticone; divinylbenzene; geranic acid; geraniol; hexanol; linalool; methanol; nerol; phenethyl alcohol; polyacrylic acid; terpineol; trans furan linalool oxide; trans rose oxide; unclassified drug; vanillin, article; chemometrics; food analysis; hydrolysis; hydrophilicity; immersion; limit of detection; limit of quantitation; mass fragmentography; mathematical analysis; priority journal; process optimization; quantitative analysis; solid phase microextraction; statistical significance; validation study; wine, Chemometrics; Gas chromatography-mass spectrometry; Primary volatile aroma compounds; Solid-phase microextraction; Validation; Wine, Gas Chromatography-Mass Spectrometry; Greece; Limit of Detection; Reference Standards; Solid Phase Microextraction; Wine
DOI:
10.1016/j.chroma.2013.07.005
