Τίτλος:
miRNA polymorphisms and risk of premature coronary artery disease
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Objective: Several microRNA (miRNA) polymorphisms have been associated
with susceptibility to specific health disorders, including
cardiovascular diseases. The aim of the present study was to investigate
whether four well-studied miRNA polymorphisms in non-Caucasian
populations, namely miR146a G>C (rs2910164), miR149 C>T (rs2292832),
miR196a2 C>T (rs11614913) and miR499 A>G (rs3746444), contribute to the
risk for the development of premature Coronary Artery Disease (CAD) in
the Greek population.
Methods: We used a case-control study to examine these associations in
400 individuals: 200 CAD patients [including a subgroup of myocardial
infraction (MI) patients] and 200 healthy controls, all of Greek origin.
MiRNA polymorphisms were genotyped using three different assays:
Polymerase chain reaction restriction fragment length polymorphism
(PCR-RFLP), High resolution Melting (HRM) and Sanger sequencing.
Results: Two of these polymorphisms, miR196a2 C>T (rs11614913) and
miR499 A>G (rs3746444) were found to be strongly associated with
increased risk for CAD (p=0.0388 and p=0.0013, respectively) and for MI
(p=0.0281 and p=0.0273, respectively). Furthermore,
miR146C-miR149C-miR196T-miR499G allele combination appeared to be
significantly related to CAD (p=0.0185) and MI (p=0.0337) prevalence.
Conclusions: Our results suggest that at least two of the studied
polymorphisms, miR196a2 C>T (rs11614913) and miR499 A>G (rs3746444), as
well as the miR146C-miR149C-miR196T-miR499G allele combination could
represent useful biomarkers of CAD and/or MI susceptibility in the Greek
population. These special genetic characteristics, in combination with
environmental factors and personal habits, might contribute to CAD
and/or MI prevalence. (C) 2020 Hellenic Society of Cardiology.
Publishing services by Elsevier B.V.
Συγγραφείς:
Agiannitopoulos, Konstantinos
Samara, Pinelopi
Papadopoulou,
Miranta
Efthymiadou, Astradeni
Papadopoulou, Eirini and
Tsaousis, Georgios N.
Mertzanos, George
Babalis, Dimitrios and
Lamnissou, Klea
Περιοδικό:
Ελληνική καρδιολογική επιθεώρηση
Εκδότης:
HELLENIC CARDIOLOGICAL SOC
Λέξεις-κλειδιά:
Coronary Artery Disease (CAD); Myocardial Infarction (MI); microRNAs
(miRNAs); polymorphisms; risk factor
DOI:
10.1016/j.hjc.2020.01.005
