Τίτλος:
Phospholipase C signaling involvement in macrotubule assembly and activation of the mechanism regulating protoplast volume in plasmolyzed root cells of Triticum turgidum
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
• The role of phosphoinositide-specific phospholipase C (PI-PLC) signaling in the macrotubule-dependent protoplast volume regulation in plasmolyzed root cells of Triticum turgidum was investigated. • At the onset of hyperosmotic stress, PI-PLC activation was documented. Inhibition of PI-PLC activity by U73122 blocked tubulin macrotubule formation in plasmolyzed cells and their protoplast volume regulatory mechanism. In neomycin-treated plasmolyzed cells, macrotubule formation and protoplast volume regulation were not affected. In these cells the PI-PLC pathway is down-regulated as neomycin sequesters the PI-PLC substrate, 4,5-diphosphate-phosphatidyl inositol (PtdInsP2). These phenomena were unaffected by R59022, an inhibitor of phosphatidic acic (PA) production via the PLC pathway. • Taxol, a microtubule (MT) stabilizer, inhibited the hyperosmotic activation of PI-PLC, but oryzalin, which disorganized MTs, triggered PI-PLC activity. Taxol prevented macrotubule formation and inhibited the mechanism regulating the volume of the plasmolyzed protoplast. Neomycin partly relieved some of the taxol effects. • These data suggest that PtdInspP2 turnover via PI-PLC assists macrotubule formation and activation of the mechanism regulating the plasmolyzed protoplast volume; and the massive disorganization of MTs that is carried out at the onset of hyperosmotic treatment triggers the activation of this mechanism. © The Authors (2008).
Συγγραφείς:
Komis, G.
Galatis, B.
Quader, H.
Galanopoulou, D.
Apostolakos, P.
Περιοδικό:
The New Phytologist
Λέξεις-κλειδιά:
1 (6 ((3 methoxyestra 1,3,5(10) trien 17 yl)amino)hexyl) 1H pyrrole 2,5 dione; 1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione; 2 pyrrolidone derivative; 6 [2 [4 [(4 fluorophenyl)phenylmethylene] 1 piperidyl]ethyl] 7 methyl 5h thiazolo[3,2 a]pyrimidin 5 one; diacylglycerol kinase; estrane derivative; mannitol; neomycin; paclitaxel; phosphatidylinositol 4,5 bisphosphate phosphodiesterase; pyrimidinone derivative; thiazole derivative; unclassified drug, cytology; cytoplasm; enzyme activity; inhibitor; osmosis; protein; root system; wheat, article; cytology; dose response; drug antagonism; drug effect; metabolism; microtubule; physiology; plant root; protoplast; signal transduction; time; wheat, Diacylglycerol Kinase; Dose-Response Relationship, Drug; Estrenes; Mannitol; Microtubules; Neomycin; Paclitaxel; Phosphoinositide Phospholipase C; Plant Roots; Protoplasts; Pyrimidinones; Pyrrolidinones; Signal Transduction; Thiazoles; Time Factors; Triticum, Triticum aestivum; Triticum turgidum
DOI:
10.1111/j.1469-8137.2007.02363.x
