Τίτλος:
Identification of fetal nucleated red blood cells in the maternal
circulation during pregnancy using anti-hemoglobin-epsilon antibody
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Aim: To investigate the use of anti-hemoglobin-epsilon, antibody in
order to identify fetal cells in the maternal circulation during
pregnancy. Materials and Methods: 48 blood samples were obtained from
pregnant women, 26 in the 1st trimester and 22 in the 2nd trimester.
Magnetic activated cell sorting was used for fetal cell enrichment
followed by immunophenotyping with a monoclonal antibody against
hemoglobin-epsilon. FISH with X, Y and 21 chromosome-specific probes was
performed in 29 cases. Results: The mean number of epsilon-positive
cells was 9.2 (range 2-23) in the 1st trimester, 4.8 (range 3-13) in the
2nd trimester and 22 (range 15-28) in pregnancies with Down syndrome. No
significant difference was noted in the number of c-positive nucleated
red blood cells (NRBCs) isolated from carriers and noncarriers of
beta-thalassemia. FISH analysis was successful in 24 cases. In 4 cases
with known male fetuses, an average of 4.7 epsilon-positive cells showed
a Y signal. In 4 cases with Down syndrome, all F-positive cells showed 3
signals for chromosome 21. Conclusion: Anti-hemoglobin-epsilon, antibody
has increased specificity for fetal NRBCs and should be preferentially
used to improve noninvasive prenatal diagnosis of chromosome
abnormalities from fetal cells in maternal blood. Copyright (C) 2003 S.
Karger AG, Basel.
Συγγραφείς:
Mavrou, A
Kolialexi, A
Antsaklis, A
Korantzis, A and
Metaxotou, C
Περιοδικό:
Fetal Diagnosis and Therapy
Λέξεις-κλειδιά:
noninvasive prenatal diagnosis; anti-epsilon antibody; fetal NRBCs;
fetal hemoglobins
