Τίτλος:
Oxidative stress in ageing and disease development studied by FT-IR spectroscopy
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
FT-IR spectroscopy was used to investigate the effect of oxidative stress and to approach the mechanism on cancer bone demineralization, aortic valve mineralization and heterotopic ossification on disease development. The FT-IR spectra obtained from paediatric, adult bone and ex vivo irradiated adult healthy bone with a dose of 20 Gy were compared with those of healthy bone. The increase of band intensity changes of vasCH2 vsCH2 in the region 3000–2850 cm−1 depended on aging, the disease progression and the dose of irradiation. The bands at 3080 cm−1 and 1744 cm−1, which originate from olefinic terminal bond (v = CH) and ester carbonyl group (vRO–C[dbnd]O), respectively, indicate the influence of oxidative stress on lipid degradation and peroxidation, respectively. The new bands at about 1690 cm−1 and 1516 cm−1 denote the presence of β-sheet conformation of the proteins due to the diseases, confirming the increasing amount of lipophilic environment and fibril formation. Comparison of the FT-IR spectra of calcified aortic valve and hip heterotopic ossification with that of normal bones showed that in the bone-like formation the peroxide anion free radicals play an important role in the disease. © 2017 Elsevier B.V.
Συγγραφείς:
Anastassopoulou, J.
Kyriakidou, M.
Kyriazis, S.
Mavrogenis, A.F.
Mamareli, V.
Mamarelis, I.
Petra, M.
Malesiou, E.
Kotoulas, C.
Kolovou, P.
Koutoulakis, E.
Markouizou, A.
Theophanides, T.
Περιοδικό:
Mechanisms of Ageing and Development
Εκδότης:
Elsevier Ireland Ltd
Λέξεις-κλειδιά:
carbonyl derivative; peroxide, adult; aortic valve; Article; beta sheet; bone biopsy; bone cancer; bone malformation; child; collagen fibril; controlled study; disease course; disease exacerbation; ex vivo study; Fourier transform infrared spectroscopy; human; human tissue; irradiation; lipid degradation; lipid peroxidation; lipophilicity; oxidative stress; priority journal; protein conformation; radiation dose; aging; aortic valve stenosis; bone tumor; calcinosis; female; femoral head; heterotopic ossification; infrared spectroscopy; male; metabolism; pathology; preschool child, Adult; Aging; Aortic Valve; Aortic Valve Stenosis; Bone Neoplasms; Calcinosis; Child; Child, Preschool; Female; Femur Head; Humans; Male; Ossification, Heterotopic; Oxidative Stress; Spectroscopy, Fourier Transform Infrared
DOI:
10.1016/j.mad.2017.11.009
