Τίτλος:
CD3-CD16-CD56bright immunoregulatory NK cells are increased in the tumor microenvironment and inversely correlate with advanced stages in patients with papillary thyroid cancer
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Background: The innate immune system is the first line of defense and plays a key role in thyroid cancer development. The role of the tumor-infiltrating natural killer (NK) cells is becoming increasingly important in research and potential cancer therapies. NK cell subpopulations, CD3-CD16 +CD56dim and CD3-CD16-CD56 bright, demonstrate a significant role in the tumor immuno-surveillance process. Methods: We investigated the distribution of CD3-CD16+CD56dim and CD3-CD16 -CD56bright NK subpopulations in tissue and blood samples from patients with papillary thyroid cancer (PTC) and nodular goiter (NG). Twenty-eight patients with PTC, 13 patients with NG, and 50 healthy donors were included in the study. Tissue and blood samples from all patients and blood samples from healthy donors were analyzed for CD3-CD16 +CD56dim and CD3-CD16-CD56 bright NK cells by flow cytometry. Results: A significant predominance of CD3-CD16+CD56dim cells compared to CD3-CD16-CD56bright NK cells was found in blood samples in all groups (p<0.0001 in PTC, NG, and healthy donors). Increased infiltration by CD3-CD16-CD56bright NK cells was observed in thyroid tissue of patients with PTC, as compared to CD3-CD16+CD56dim NK cells (p=0.046), while CD3-CD16+CD56dim NK cells demonstrated a higher infiltration of NG tissues. CD3-CD16+CD56dim NK cell tissue infiltration positively correlated with advanced stages of PTC. In contrast, the CD3-CD16-CD56bright NK cell population was negatively associated with tumor stage in patients with PTC. Conclusion: CD3-CD16-CD56bright NK cell infiltration seems to be associated with PTC progression. These findings contribute to a better understanding of the immune response in PTC and may lead to novel immunotherapeutic approaches in these patients. © Copyright 2013, Mary Ann Liebert, Inc. 2013.
Συγγραφείς:
Gogali, F.
Paterakis, G.
Rassidakis, G.Z.
Liakou, C.I.
Liapi, C.
Περιοδικό:
Thyroid Research
Λέξεις-κλειδιά:
adult; advanced cancer; aged; article; blood sampling; cell infiltration; cellular distribution; clinical article; controlled study; disease severity; female; human; human cell; innate immunity; male; natural killer cell; nodular goiter; priority journal; thyroid carcinoma; thyroidectomy; tumor immunity; tumor microenvironment, Adolescent; Adult; Aged; Carcinoma, Papillary; Female; Flow Cytometry; Humans; Killer Cells, Natural; Male; Middle Aged; Neoplasm Staging; Thyroid Neoplasms; Tumor Microenvironment; Young Adult
DOI:
10.1089/thy.2012.0560
