Τίτλος:
HOXA9 and MEIS1 gene overexpression in the diagnosis of childhood acute leukemias: Significant correlation with relapse and overall survival
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Homeobox genes HOXA9 and MEIS1 are evolutionarily conserved transcription factors with essential roles in both hematopoiesis and leukemogenesis. They act as dominant cooperating oncoproteins that cause acute leukemias bearing MLL translocations and to a lesser extent T-cell acute lymphocytic leukemia (ALL) characterized by other gene fusions. Overexpression is associated with an adverse prognosis in adults. In childhood, the genes have only been investigated in leukemias bearing MLL translocations. The aim of this study was to determine whether overexpression extends to leukemic subtypes other than the MLL-positive subtype in childhood. We use quantitative real-time PCR methodology to investigate gene expression in 100 children with acute leukemias and compare them to those of healthy controls. We show that abnormally high HOXA9 and MEIS1 gene expression is associated with a variety of leukemic subtypes, including various maturation stages of B-cell ALL and cytogenetic types other than the MLL-positive population, thus suggesting that the genes are implicated in the development of a broad range of leukemic subtypes in childhood. In addition, we show that HOXA9 and MEIS1 overexpression are inversely correlated with relapse and overall survival, so the genes could become useful predictive markers of the clinical course of pediatric acute leukemias. © 2015 Elsevier Ltd.
Συγγραφείς:
Adamaki, M.
Lambrou, G.I.
Athanasiadou, A.
Vlahopoulos, S.
Papavassiliou, A.G.
Moschovi, M.
Περιοδικό:
Leukemia Research
Εκδότης:
Elsevier Ireland Ltd
Λέξεις-κλειδιά:
transcription factor HoxA9; homeodomain protein; myeloid ecotropic viral integration site 1 protein; transcription factor HoxA9; tumor marker; tumor protein, acute leukemia; acute lymphoblastic leukemia; acute myeloblastic leukemia; Article; B cell lymphoma; Burkitt lymphoma; cancer diagnosis; cancer prognosis; cancer survival; cell maturation; child; childhood leukemia; controlled study; correlational study; cytogenetics; disease free survival; drug treatment failure; female; gene; gene overexpression; gene translocation; HOXA9 gene; human; human cell; human tissue; leukemia cell line; leukemia free time; leukemia relapse; major clinical study; male; MEIS1 gene; minimal residual disease; overall survival; preschool child; priority journal; quantitative analysis; school child; survival prediction; T cell leukemia; adolescent; gene expression regulation; genetics; infant; mortality; pathology; prognosis; recurrent disease; survival; upregulation, Adolescent; Child; Child, Preschool; Female; Gene Expression Regulation, Leukemic; Homeodomain Proteins; Humans; Infant; Male; Neoplasm Proteins; Precursor Cell Lymphoblastic Leukemia-Lymphoma; Prognosis; Recurrence; Survival Analysis; Tumor Markers, Biological; Up-Regulation
DOI:
10.1016/j.leukres.2015.04.012
