Τίτλος:
Cdc6 expression represses E-cadherin transcription and activates adjacent replication origins
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
E-cadherin (CDH1) loss occurs frequently in carcinogenesis, contributing to invasion and metastasis. We observed that mouse and human epithelial cell lines overexpressing the replication licensing factor Cdc6 underwent phenotypic changes with mesenchymal features and loss of E-cadherin. Analysis in various types of human cancer revealed a strong correlation between increased Cdc6 expression and reduced E-cadherin levels. Prompted by these findings, we discovered that Cdc6 repressed CDH1 transcription by binding to the E-boxes of its promoter, leading to dissociation of the chromosomal insulator CTCF, displacement of the histone variant H2A.Z, and promoter heterochromatinization. Mutational analysis identified the Walker B motif and C-terminal region of Cdc6 as essential for CDH1 transcriptional suppression. Strikingly, CTCF displacement resulted in activation of adjacent origins of replication. These data demonstrate that Cdc6 acts as a molecular switch at the E-cadherin locus, linking transcriptional repression to activation of replication, and provide a telling example of how replication licensing factors could usurp alternative programs to fulfill distinct cellular functions. © 2011 Sideridou et al.
Συγγραφείς:
Sideridou, M.
Zakopoulou, R.
Evangelou, K.
Liontos, M.
Kotsinas, A.
Rampakakis, E.
Gagos, S.
Kahata, K.
Grabusic, K.
Gkouskou, K.
Trougakos, I.P.
Kolettas, E.
Georgakilas, A.G.
Volarevic, S.
Eliopoulos, A.G.
Zannis-Hadjopoulos, M.
Moustakas, A.
Gorgoulis, V.G.
Περιοδικό:
JOURNAL OF CELL BIOLOGY
Λέξεις-κλειδιά:
cell cycle protein 6; histone H2AZ; neurogenic differentiation factor; transcription factor CTCF; uvomorulin, animal cell; animal experiment; animal model; animal tissue; article; carboxy terminal sequence; carcinogenesis; controlled study; dissociation; DNA replication; E box element; epithelium cell; female; gene expression; gene locus; gene overexpression; gene repression; genetic linkage; genetic variability; heterochromatin; heterochromatization; human; human cell; male; mesenchyme cell; mouse; mutational analysis; neoplasm; nonhuman; phenotype; priority journal; promoter region; protein analysis; protein binding; protein blood level; protein depletion; protein motif; transcription initiation, Amino Acid Motifs; Animals; Cadherins; Cell Cycle Proteins; Cell Line; DNA; DNA Replication; Dogs; Down-Regulation; Histones; Humans; Mice; Mice, SCID; Nuclear Proteins; Oncogenes; Promoter Regions, Genetic; Repressor Proteins; RNA, Messenger; Transcription, Genetic
DOI:
10.1083/jcb.201108121
