Τίτλος:
Biocatalytic preparation of acylated derivatives of flavonoid glycosides
enhances their antioxidant and antimicrobial activity
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Enzymatic synthesis of acylated derivatives of a monosaccharidic
flavonoid chrysoeriol-7-O-beta-D-(3”-E-p-coumaroyl)glucopyranoside as
well as of a disaccharidic flavonoid
chrysoeriol-7-[6’”-O-acetyl-beta-D-allosyl-(1 –>
2)-beta-D-glucopyranoside], isolated from Greek endemic plants, was
performed using an immobilized Candida antarctica lipase in non-toxic
organic solvents. The influence of the reaction parameters such as the
molar ratio of acyl donor to flavonoid, as well as the nature of the
acyl donor, on the performance of the biocatalytic process was pointed
out using the acylation of naringin as a model reaction. With vinyl
laurate as acyl donor, the highest conversion was observed at relatively
high molar ratio (greater than or equal to 10), using acetone as
solvent. Lipase exhibits specificity towards primary alcohol of the
glucose moiety of both flavonoid glycosides. The introduction of an acyl
group into glucosylated flavonoids significantly improved their
antioxidant activity towards both LDL and serum model in vitro.
Furthermore, the acylated derivative of disaccharidic flavonoid
increased its antimicrobial activity against two Gram-positive bacteria.
(C) 2004 Elsevier B.V. All rights reserved.
Συγγραφείς:
Mellou, F
Lazari, D
Skaltsa, H
Tselepis, AD
Kolisis, E
and Stamatis, H
Περιοδικό:
Journal of Biotechnology
Εκδότης:
ELSEVIER SCIENCE BV
Λέξεις-κλειδιά:
lipase; esterification; flavonoids; biocatalysis; LDL oxidation;
antioxidants
DOI:
10.1016/j.jbiotec.2004.12.002
