Τίτλος:
Effect of luteal-phase support on endometrial L-selectin ligand expression after recombinant follicle-stimulating hormone and ganirelix acetate for in vitro fertilization
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Context: The impact of different types of luteal phase support on endometrial receptivity after ovarian stimulation has not been investigated. Objective: Our objective was to evaluate the impact of different luteal-phase support protocols on sex steroid levels and on endometrial expression of L-selectin ligand after ovarian hyperstimulation with a GnRH antagonist protocol. Patients and Design: Seventeen oocyte donors who underwent ovarian stimulation with a recombinant FSH/ganirelix acetate protocol were randomized into three groups: group I had no luteal-phase support; group II had luteal support with micronized progesterone; and group III had luteal support with progesterone plus 17β-estradiol. All donors had endometrial biopsies on the day of retrieval, and then 3, 5, and 10 d after retrieval. In addition, they had serum estradiol and progesterone measurements on d 3, 5, and 10. Main Outcome Measures: Endometrial L-selectin ligand expression was detected by immunohistochemical staining in the luminal and glandular epithelium. A histological score was used for the quantification of the immunostaining. Sex steroid levels were measured during the luteal phase. Results: By d 10 after retrieval, there was a significant decrease in mean progesterone levels in group I compared with the other two groups that may reflect the expected demise of the corpus luteum. There was also a significant increase in the presence of L-selectin ligands in the luminal epithelium in group III. Conclusions: During controlled ovarian stimulation with a GnRH antagonist protocol, luteal-phase support with micronized progesterone and 17β-estradiol seem to increase endometrial L-selectin ligand expression in the luminal endothelium. Copyright © 2006 by The Endocrine Society.
Συγγραφείς:
Vlahos, N.F.
Lipari, C.W.
Bankowski, B.
Lai, T.-H.
King, J.A.
Shih, I.-M.
Fragakis, K.
Zhao, Y.
Περιοδικό:
JOURNAL OF CLINICAL ENDOCRINOLOGY AND METABOLISM
Εκδότης:
Japan Endocrine Society
Λέξεις-κλειδιά:
estradiol; ganirelix; L selectin; progesterone; recombinant follitropin; sex hormone, article; controlled study; corpus luteum; donor; fertilization in vitro; gene expression; human; immunohistochemistry; luteal phase; priority journal; staining
DOI:
10.1210/jc.2006-0520
