Τίτλος:
Potential role of apoptosis and apoptotic regulatory proteins in colorectal neoplasia: Correlations with clinico-pathological parameters and survival
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
An imbalance between apoptotic and proliferative processes is believed to underlie colorectal neoplasia. We evaluated the expression of bcl-2, p53, mdm2 proteins, and apoptosis in colorectal neoplasms, as well as their correlation with clinico-pathological parameters, using image analysis. Biopsies from 46 colorectal cancers, 121 adenomas, and 25 controls were studied using monoclonal antibodies against p53, bcl-2, mdm2 and the terminal deoxynucleotidyl- transferase-mediated dUTP nick end labeling (TUNEL) method for apoptosis. P53 and bcl2 protein expression was higher in adenomas ≥1 cm (P < 0.03) and tubulovillous-villous adenomas (P < 0.03), and correlated with dysplasia (P < 0.03). In Cox regression analysis, Dukes' stage was the most significant independent prognostic indicator of a worse survival (P < 0.019), whereas when stage was eliminated, bcl-2 expression was also a powerful predictor for bad prognosis (P = 0.02). In conclusion, both bcl-2 and p53 immunohistochemical profiles may be useful adjuncts in detecting adenomas with a malignant potential, whereas bcl-2 could be used in combination with Dukes' stage as a predictor of prognosis in colorectal cancer. © 2007 Springer Science+Business Media, LLC.
Συγγραφείς:
Tzouvala, M.
Lazaris, A.C.
Papatheodoridis, G.V.
Kouvidou, C.
Papathomas, T.G.
Kavantzas, N.
Elemenoglou, I.
Karamanolis, D.G.
Agapitos, E.
Περιοδικό:
Digestive Diseases and Sciences
Λέξεις-κλειδιά:
protein bcl 2; protein MDM2; protein p53, adult; aged; apoptosis; article; cancer survival; colorectal cancer; controlled study; correlation analysis; dysplasia; female; histopathology; human; human tissue; immunohistochemistry; immunophenotyping; major clinical study; male; nick end labeling; prediction; priority journal; prognosis; proportional hazards model; protein expression; villous adenoma, Adenoma; Adult; Aged; Apoptosis; Colorectal Neoplasms; Female; Humans; Image Processing, Computer-Assisted; Immunohistochemistry; Immunophenotyping; In Situ Nick-End Labeling; Male; Middle Aged; Prognosis; Proportional Hazards Models; Proto-Oncogene Proteins c-bcl-2; Proto-Oncogene Proteins c-mdm2; Tumor Suppressor Protein p53
DOI:
10.1007/s10620-007-9857-6
