Περίληψη:
Background: Fibroses of vessels and soft tissue are side effects of
radiotherapy. The authors assumed that there was an immediate direct
radiogenic damage of collagen of bone, periosteum and skin.
Material and Methods: 15 porcine jaws samples (group 1) were exposed to
a total dose of 60 Gy (cobalt-60, 2 Gy/day, five fractions/week). 15
jaws samples were stored accordingly (group 2, no irradiation, control).
Collagen fragments of bone, periosteum and skin samples of groups 1 and
2 were isolated by ultrafiltration. Collagen types were characterized by
SDS-PAGE measurement of the mature collagen cross-links
hydroxylysylpyridinoline (HP) and tysylpyridinoline (LP) by
high-performance Liquid chromatography (HPLC) and analysis of
hydroxyproline (Hyp) was used to determine the ratio of the amount of
collagen fragments from irradiated as opposed to nonirradiated samples.
Results: The concentrations of HP, LP and Hyp in ultrafiltrates of
probes of irradiated bone, periosteum and skin were markedly increased
(average factors for bone: 3.69, 1.84, and 3.40, respectively; average
factors for periosteum: 1.55, 1.41, and 1.77, respectively; average
factors for skin: 1.55, 1.60, and 2.23, respectively) as compared to
nonirradiated probes. SDS-PAGE did show collagen types I and V in
nonirradiated bone, I and III in nonirradiated skin, and I in
nonirradiated periosteum samples. In irradiated samples, smeared bands
illustrated fragmentation of the collagen molecule.
Conclusion: The increased concentrations of HP, LP and Hyp in
ultrafiltrates indicated increased concentrations of split collagen.
Direct and instant radiogenic damage of (extracellular matrix of) bone,
periosteum and skin tissue collagen could be demonstrated.
Συγγραφείς:
Acill, Yahya
Springer, Ingo N.
Niehoff, Peter
Gassling,
Volker
Warnke, Patrick H.
Acmaz, Sedat
Soenmez, Tolga Taha
and Kimmig, Bernhard
Lefteris, Vairaktaris
Wiltfang, Joerg
