Τίτλος:
OCT-4 and DAZL expression in precancerous lesions of the human uterine cervix
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Aim To determine whether octamer-binding transcription factor 4 (OCT-4) and deleted in azoospermia like (DAZL) are expressed among cells with human papilloma virus (HPV) infection and cervical intraepithelial neoplasia (CIN) lesions and quantify their relative expression when compared with normal cervical cultures. Methods Cervical cells derived from normal cell cultures, HPV lesions and CIN lesions were cultured in Dulbecco's modified Eagle's medium supplemented with 20% amniotic fluid and 5 ng/mL basic fibroblast growth factor at 37°C and humidified 10% CO2 in air. Real-time polymerase chain reaction (PCR) was carried out using G6PD as a reference. We used REST for statistical analysis of real-time PCR. Results Whereas DAZL was not expressed either in normal cultures or HPV and CIN lesions, OCT-4 was expressed in all examined cell lines. Moreover its relative expression was significantly upregulated among cultures of HPV-infected cells (RE, 11.003; 95%CI: 0.054-36 704.527, P = 0.042), an observation that was also close to statistical significance among cultures of CIN lesions (P = 0.066). Conclusion The relative expression of OCT-4 is upregulated during the early, preinvasive stages of cervical carcinogenesis. Future studies should investigate its potential as a screening marker and as a possible target of therapy. © 2014 The Authors. Journal of Obstetrics and Gynaecology Research © 2014 Japan Society of Obstetrics and Gynecology.
Συγγραφείς:
Stefanidis, K.
Pergialiotis, V.
Christakis, D.
Patta, J.
Stefanidi, D.
Loutradis, D.
Περιοδικό:
Journal of Obstetrics and Gynaecology Research
Λέξεις-κλειδιά:
deleted in azoospermia like protein; fibroblast growth factor 2; glucose 6 phosphate dehydrogenase; octamer transcription factor 4; peptides and proteins; unclassified drug; DAZL protein, human; octamer transcription factor 4; RNA binding protein; tumor marker, amnion fluid; Article; cancer stem cell; carcinogenesis; clinical article; controlled study; female; human; human cell; human tissue; papillomavirus infection; protein expression; uterine cervix carcinoma in situ; uterine cervix cytology; gene expression regulation; metabolism; pathology; tumor cell line; upregulation; uterine cervix; uterine cervix carcinoma in situ; uterine cervix tumor; virology, Biomarkers, Tumor; Cell Line, Tumor; Cervical Intraepithelial Neoplasia; Cervix Uteri; Female; Gene Expression Regulation, Neoplastic; Humans; Octamer Transcription Factor-3; Papillomavirus Infections; RNA-Binding Proteins; Up-Regulation; Uterine Cervical Neoplasms
