Τίτλος:
Proteomic analysis of pubocervical fascia in women with and without
pelvic organ prolapse and urodynamic stress incontinence
Γλώσσες Τεκμηρίου:
Αγγλικά
Περίληψη:
Introduction and hypothesis This pilot study compares protein expression
patterns in the pubocervical fascia of women with pelvic organ prolapse
(POP) and stress urinary incontinence (SUI) and asymptomatic women with
normal pelvic support
methods Samples of pubocervical fascia were collected from four women
with POP and SUI and from three asymptomatic (control) women These were
analyzed using two-dimensional electrophoresis and matrix-assisted laser
desorption/ionization time-of-flight mass spectrometry.
Results The expression levels of transgelin, smooth muscle gamma-actin,
myosin light polypeptide 6, and alpha-1 antitrypsin precursor were more
than twofold higher in patients than in controls An additional five
proteins were overexpressed (more than twofold) in patients, while three
proteins were detected only in the patients.
Conclusion This pilot study is the first study to estimate changes in
protein expression in the pubocervical fascia of human patients with
POP. These changes could be related to the pathophysiology of POP
Συγγραφείς:
Athanasiou, Stavros
Lymberopoulos, Elias
Kanellopoulou, Sofia
and Rodolakis, Alexandros
Vlachos, George
Antsaklis, Aris
Περιοδικό:
INTERNATIONAL UROGYNECOLOGY JOURNAL AND PELVIC FLOOR DYSFUNCTION
Εκδότης:
Springer-Verlag London Ltd
Λέξεις-κλειδιά:
MALDI-TOF-MS; Mass spectrometry; Pelvic organ prolapse; Proteomics;
Pubocervical fascia; Stress urinary incontinence; Two-dimensional
electrophoresis
DOI:
10.1007/s00192-010-1203-4
