Unit:
Τομέας ΠαθολογίαςLibrary of the School of Health Sciences
Author:
Κουσκούκης Αλέξανδρος Ευστράτιος
Dissertation committee:
Ρηγόπουλος Δημήτριος, Κατσάμπας Ανδρέας, Γεωργάλα Σοφία
Original Title:
Ανίχνευση του ιού των ανθρωπίνων θηλωμάτων (HPV) σε ιστολογικά παρασκευάσματα διαφόρων κλινικών μορφών δερματικού κακοήθους μελανώματος στον ελληνικό πληθυσμό
Summary:
In this dissertation the correlation between the existence of human papilloma
virus HPV in histological preparations of cutaneous malignant melanoma in
Greek population was investigated. Indications in other research studies
encouraged the accomplishment of this research, using classic and contemporary
HPV detection methods and techniques.
Cutaneous malignant melanoma is a very aggressive disease which has very low
life expectancy and it occurs due to many reasons such as genetic
predisposition, environmental factors as exposure to UVA/UVB radiation and
other parameters. This cancer develops due to mutations in pre-oncogenes and
suppressor genes which have cumulative impact on human organism. Final result
of this cumulative act is the failure of cell repair mechanisms to repair the
total damage caused to the cells and the development of cutaneous malignant
melanoma.
There are several ways to confront the cutaneous malignant melanoma but the
disease is not curable after a certain point of development. The basic tools
that nowadays doctors have to confront the cutaneous malignant melanoma is the
prognosis, the prevention as well as the surgical extraction, the biological
therapies and the chemotherapies or more often a combination of the above.
The material and techniques used for the isolation of HPV from parafin embedded
histological preparations of cutaneous malignant melanoma was a DNA extraction
kit, which used proteinase K. DNA samples were amplified by primers MY09, MY11
and PCR and the PCR products were transferred to agarose gels, where current
was applied. Finally, the gels were photographed under a transilluminator
device.
Having used the techniques of the protocol, the results of the research showed
that HPV was not detected in the histological preparations. 32 samples from
cutaneous malignant melanoma histological preparations were analysed. The age
class of the participant patients was 22-92 years old and malignant melanoma
was found in various body sites and in the head. 37,5% of the samples were
female and 62,5% were male.
The conclusion from the study is that there is no correlation of human
papilloma virus (HPV) and cutaneous malignant melanoma in the histological
preparations of Greek population analysed, although previous studies showed
indications of HPV and cutaneous malignant melanoma correlation. The most
significant parameter of malignant melanoma development remains UV radiation,
mutations in CDKN2A gene with the presence of MC1R gene polymorphisms, but
further investigation of the etiology of cutaneous malignant melanoma is
necessary.
Keywords:
human papilloma, malignant melanoma, HPV virus, oncogene, suppressor genes
Number of references:
157
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