Τομέας Κλινικοεργαστηριακός
Library of the School of Health Sciences

2014-09-19

2014

Γαβαλά Αλεξάνδρα

Κίττας Χρήστος, Μπαλτόπουλος Γεώργιος, Αλεβιζόπουλος Γεώργιος

Ανοσιακή απόκριση υγιών μετά από οξεία έκθεση σε αλκοόλη

Greek

Immune response in healthy volunteers after acute exposure to alcohol

The purpose of the study was to investigate the effect of acute exposure to
alcohol on immune response, via the whole blood cytokine production after LPS
stimulation ex-vivo.
Methods: Whole blood was taken from healthy volunteers and was placed in tubes
containing EDTA. Blood samples were immediately diluted 1:10 in RPMI 1640
culture medium. LPS (500 pg) was added, after pretreatment with six different
alcohol doses (final concentrations 5‚ 12.5‚ 25‚ 50‚ 100 and 200mM) for 10
minutes, according to the study protocol. After incubation, samples were
centrifuged and culture supernatants were collected for measurement of TNFa, ΙL-
6, ΙL-10, sTNFR Ι and sTNFR ΙΙ levels.
Results: We studied 24 healthy male volunteers aged 36.5 ± 1.4 (X ± SEM).
Alcohol caused a dose dependent decrease of TNF-α and IL-10 production after
LPS stimulation. Alcohol at lower doses (<50mM) seemed to decrease TNFR II
levels, but an increase was observed at higher doses of alcohol (100 and 200
mM), which was statistically significant after LPS stimulation ex vivo.
Conclusions: Alcohol has a suppressive effect on both pro-inflammatory and
anti-inflammatory responses, and a differential effect on sTNFR II whole blood
production with or without LPS stimulation ex-vivo, depending on the alcohol
concentration.

Acute exposure to alcohol, Whole blood, Stimulation with lipopolysacharide, Cytokines, Soluble TNF-α receptors

No

0

Yes

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https://pergamos.lib.uoa.gr/uoa/dl/object/1305714