Unit:
Τομέας ΧειρουργικήςLibrary of the School of Health Sciences
Author:
Μουλαβασίλη Παναγιώτα
Dissertation committee:
Καθηγητής κ. Ελ. Βαϊρακτάρης, Καθηγήτρια κ. Δέσποινα Ν. Περρέα, Επ. Καθηγητής κ. Δ. Ηλιόπουλος
Original Title:
Ανοσοϊστοχημική μελέτη σε επίμυες των γονιδίων ΝΕU και N-ras , που σχετίζονται με μηχανισμούς ογκογένεσης στη στοματική κοιλότητα
Translated title:
Immunohistochemical study in hamsters of the genes NEU and N-RAS ,which are related to the oral oncogenesis process
Summary:
This study was designed to determine the gradual expression of the oncogenes
neu and N-ras and their feasible correlation in an experimental model of
chemically induced oral carcinogenesis. 37 Syrian golden hamsters were divided
into three experimental groups and one control group. The left buccal pouches
of animals in experimental groups were painted for 14 weeks with carcinogen
DMBA and were excised at 10, 14, 19 weeks respectively. The biopsies were
classified pathologically and were studied immunohistochemically. Statistical
analysis was then performed. A gradual increase of neu expression from oral
mucosa to non-cancerous and precancerous stages was observed, while in the
initial tumour stages the expression remained almost stable. Interestingly, the
neu cytoplasmic staining was very elevated compared to membrane staining in the
initial stages. The expression of N-ras gradually decreased during oral
carcinogenesis. The observed patterns of neu and N-ras expression do not seem
to correlate. Conclusively, there is a strong correlation between neu
overexpression and oral cancer progression. Cytoplasmic neu expression may be
useful as an early prognostic factor. A signalling pathway not involving N-ras
may be implicated in the oral oncogenesis. The existing pathway implicating
the proteins neu and N-ras may be somehow altered in oral cancer.
Keywords:
Genes Neu, N-ras, Oral cancer, Immunohistochemistry, Syrian golden hamsters, Chemical induced carcinogenesis, carcinogen DMBA
Number of references:
316
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