Τομέας Κλινικοεργαστηριακός
Library of the School of Health Sciences

2016-09-19

2016

Κατσάρης Ματθαίος

Μ. Χρυσοφός: Αναπλ. Καθηγητής, Επιβλέπων Μέλος ΔΕΠ

Ανοσοπαρεμβατική θεραπεία με ιντερφερόνη - γάμμα σε πειραματική πυελονεφρίτιδα από escherichia coli

Greek

IMMUNOMODULATORY INTERVENTION WITH INTERFERON-GAMMA IN EXPERIMENTAL ESCHERICHIA COLI PYELONEPHRITIS

Objective: To investigate the efficacy of recombinant human interferon gamma
(rhIFNβ) in experimental pyelonephritis by Escherichia coli.
Methods: Pyelonephritis was induced by intrapelvic inoculation of bacteria
after ureteral ligation in 38 rabbits assigned in three groups: group A (n= 16)
controls (n=12); group B (n= 14) treated with intravenous rhIFNβ; and group C
(n= 8) treated with both intravenous rhIFNβ and amikacin. Bacterial counts,
cytokines and malondialdehyde (MDA) were measured in blood. Peripheral blood
mononuclear cells (PBMCs) were isolated for measurement of TNFα
transcripts, cytokine stimulation and measurement of apoptosis. Survival was
recorded and tissue bacterial load and myeloperoxidase (MPO) activity after
sacrifice were measured.
Results: Mortality of group A was 66.7%, of group B 25% and of group C 12.5%.
Circulating bacterial counts and tissue bacterial load of group B were lower
than group A. Negative correlation was found between circulating MDA and the
bacterial load of the spleen. Although the number of TNFα transcripts in
circulating PBMCs did not differ, PBMCs isolated from group B at 48 hours
produced much greater concentrations of TNFα after stimulation with Pam3Cys.
In parallel, the rate of apoptosis of circulating monocytes was increased in
group B at 48 hours. MPO activity of the lung after 24 hours, used as indirect
evidence of neutrophil infiltration, was decreased in group B.
Conclusions: Administration of rhIFNβ prolonged survival in experimental
urosespsis by E.coli. Its action is probably related with increased bacterial
phagocytosis after modulation of the oxidant status and reversal of
immunoparalysis of monocytes.

Interferon-gamma, Survival, Urosepsis, Anosoparalysis, Monocytes

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https://pergamos.lib.uoa.gr/uoa/dl/object/1305756