Unit:
Τομέας ΚλινικοεργαστηριακόςLibrary of the School of Health Sciences
Author:
Παπαβασιλείου Κωνσταντίνα-Δήμητρα
Dissertation committee:
Αναπληρωτής Καθηγητής Στυλιανός Χατζηπαναγιώτου,
Original Title:
Διερεύνηση της παραγωγής βιομεμβρανών και της παρουσίας του λοιμογόνου παράγοντα Τ3S (σύστημα έκκρισεως τύπου ΙΙΙ) σε κλινικά στελέχη Salmonella που απομονώθηκαν σε παιδιά.
Summary:
The purpose was to investigate the presence of virulence factors (biofilms,
virulence genes) and compare their antimicrobial resistance in association with
their biofilm forms and the presence of the virulence genes.
From 2006 to 2008, 194 Salmonella strains were collected at the Pentelis
Children’s Hospital from children with gastroenteritis. Isolation and
serological identification was performed by conventional methods. Antimicrobial
susceptibility was performed by means of determination of the minimum
inhibitory concentration (MIC) with the automatic VITEK 2 system
(bioMerieux-France) and by the standard broth dilution method. Biofilm
formation was detected by the use of silicone disks. The strains producing
biofilms were tested for antimicrobial susceptibility using a modified broth
macrodilution method. All strains were screened for the presence of virulence
genes using a PCR assay. A statistical package SPSS was used.
Of the 194 Salmonella Enterica isolates, 19 different serotypes were
identified. S.Enteritidis was the predominant serotype. 56% were positive for
biofilm formation. The biofilm forms showed increased antimicrobial resistance.
At least one virulence gene was present in all strains. Statistical analysis
showed that there was a significant association regarding the presence of the
virulence genes. The ability to form biofilm was statistically independent of
any of the six genes.
Keywords:
Biofilms, Genes, T3SS, Antibiotics, Salmonella
Number of references:
190
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