Τομέας Φυσιολογίας Ζώων και Ανθρώπου
Library of the School of Science

2014-02-06

2014

Κλουκίνα-Πανταζίδου Ισμήνη

Γαϊτανάκη Αικατερίνη Καθηγήτρια ΕΚΠΑ (επιβλέπουσα), Σπύρος Ευθυμιόπουλος Αναπλ. Καθηγητής ΕΚΠΑ, Μαριέττα Ισιδωρίδου Ράδοβιτς Καθηγήτρια Πανεπιστημίου Ιλλινόις ΗΠΑ

Ιστολογική και Υπερμικροσκοπική Ανοσοεντόπιση των Ενζύμων και Πρωτεϊνών των Κυτταρικών Δομών Αποθήκευσης της Νοραδρεναλίνης στον Κατεχολαμινικό Πυρήνα Locus Coeruleus του Ανθρώπινου Εγκεφάλου

Greek

Histological and Ultrastructural Immunolocalization of Enzymes and Proteins of Noradrenaline-storing Organelles in Human Catecholamine Locus Coeruleus Neurons

Human monoamine neurons are characterized by abundant spherical acidophilic
protein-bodies, which originate from mitochondria, retaining the double
membrane. In Locus Coeruleus (LC), they have somatodendritic distribution and
are unequivocal storage vesicles for noradrenaline. Their depletion is
accompanied by Parkinson’s disease (PD) pathology. The aim of this study was to
investigate their composition and role in normal and pathological conditions.
Post-mortem brain specimens were collected from control subjects and PD
patients. Human adrenal medulla was used as a model-tissue and histochemical
and immunohistochemical correlation between protein-bodies and chromaffin
granules was made. At the ultrastructural level, the colloidal immunogold
method was used. The mitochondrial origin of protein-bodies was sealed with
their positive immunoreactivity for mitochondrial porin. The data provided
demonstrate the ultrastructural immunolocalization of both Chromogranin-A and
Vesicular Monoamine Transporter-2 in protein-bodies. The study of pathological
brains also revealed the localization of a-synuclein in protein-bodies, as well
as, in neuromelanin of LC neurons. The study of protein-bodies, which are
responsible for somatodentritic storage and possible release of noradrenaline
in human LC neurons, and their contribution in the formation of Lewy bodies, as
indicated by the localization of common components among these two structures
may be helpful, towards the understanding of PD.

Locus Coeruleus, Protein Bodies, Adrenal Medulla, Electron Microscopy, Parkinson's Disease

No

0

Yes

230

265

File access is restricted only to the intranet of UoA.

https://pergamos.lib.uoa.gr/uoa/dl/object/1308963