Unit:
Κατεύθυνση Ανόργανη Χημεία και ΤεχνολογίαLibrary of the School of Science
Author:
Μαυροειδή Βαρβάρα
Dissertation committee:
Κων/νος Μεθενίτης, Αναπλ. Καθηγητής ΕΚΠΑ (Επιβλέπων), Μαρία Πελεκάνου Ερευνήτρια Α΄, ΕΚΕΦΕ ΔΗΜΟΚΡΙΤΟΣ, Αθανάσιος Φιλιππόπουλος Επίκ. Καθηγητής ΕΚΠΑ,
Original Title:
Σύνθεση και χαρακτηρισμός συμπλόκων στοιχείων μεταπτώσεως με παράγωγα του βενζοθειαζολίου. Μελέτη της αλληλεπίδρασης των συμπλόκων με νουκλεοτίδια. In vitro διερεύνηση της βιολογικής τους δράσης
Translated title:
Synthesis and characterization of complexes of transition elements with benzothiazole derivatives. Study of the interaction of the complexes with nucleotides. In vitro investigation of their biological activity.
Summary:
In the present thesis two ligands, bearing the antitumor unit of 2-(4΄-
aminophenyl)benzothiazole bonded through a hydrocarbon chain with
2-aminomethylpyridine, were synthesized. Their corresponding Pd(II), Pt(II) and
Cu(II) complexes were successfully synthesized and fully characterized by
various spectroscopic methods (IR, NMR, MS, UV-Vis and EPR). In addition, their
binding to CT-DNA was also been evaluated by UV-Vis spectroscopy, fluorescence,
circular dichroism, viscometry and thermal denaturation studies. The data
reveal that both ligands and complexes strongly interact with CT-DNA. Βoth
ligands interact with CT-DNA via a combined mode of action involving both
groove binding and non-classical intercalation, while in the case of the
complexes covalent bond formation takes place as well. Finally, their in vitro
biological activity was been investigated using two human breast cancer cell
lines (MCF-7 and MDA-MB 231) as well as healthy human skin fibroblasts (DSF).
The encouraging preliminary in vitro biological results show uptake of the
intact complexes by the breast cancer cells and increased selective and
activity.
Keywords:
2-(4'-Aminophenyl)benzothiazole , Platinum(II), Palladium(II), DNA, Anticancer activity
Number of index pages:
1-9
Number of references:
184
