Unit:
Κατεύθυνση Αναπαραγωγική-Αναγεννητική ΙατρικήLibrary of the School of Health Sciences
Supervisors info:
Δημήτριος Λουτράδης, Κωνσταντίνος Καλλιανίδης, Κωνσταντίνος Στεφανίδης
Original Title:
Η δράση της μετφορμίνης στην in vitro ανάπτυξη ωοθυλακιών επιμύων μετά από μακράς διάρκειας καλλιέργειες.
Translated title:
The role of metformin in murine follicular oocyte maturation in long term culture system.
Summary:
Metformin is an oral antidiabetic drug in the biguanide class. It is the
first-line drug
of choice for the treatment of type 2 diabetes, suppressing glucose production
by the liver.
Metformin is also used in the treatment of polycystic ovary syndrome, since
patients with
that syndrome may indicate impaired glucose tolerance (insulin resistance).
Metformin activates AMP-activated protein kinase (AMPK), an enzyme that plays
an important role in insulin signaling, whole body energy balance, and the
metabolism of
glucose and fats. AMPK is a serine/threonine kinase and is an heterotrimeric
protein formed
by α, β, and γ subunits. The aim of this study was to investigate the role of
metformin in murine follicular
oocyte maturation in long term culture system. It was studied the expression
pattern of
genes that encode AMPK in preantral and antral follicles and in cumulus–oocyte
complexes
(COCs) after ovulation. Furthermore, it was studied the effect of metformin in
steroidogenesis, using quantitative analysis of progesterone,
D4-androstenedione and testosterone produced during the culture period.
Keywords:
Metformin, AMPK, Maturation, Follicles, Steroidogenesis
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