Κατεύθυνση Κλινικοπαθολογοανατομική θεώρηση των νεοπλασιών του ανθρώπου
Library of the School of Health Sciences

2013-07-25

2013

Παπατρέχας Κωνσταντίνος

Αικατερίνη Παυλάκη, Αναπληρώτρια Καθηγήτρια

Τεχνικές ανίχνευσης στελεχών του ιού HPV

Greek

Human papillomaviruses (HPVs) comprise more than 100 genotypes. The mucosal
types can be divided into high-risk and low-risk (LR) types depending on the
associated disease risk. Human papillomavirus (HPV) testing can identify women
at risk of cervical cancer. HPVs cannot be cultured and the detection of virus
relies on a variety of techniques used in immunology, serology, and molecular
biology. Currently, molecular detection methods are the gold standard for
identification of HPV. The three categories of molecular assay that are
available are based on the detection of HPV DNA and include: 1) non-amplified
hybridization assays, such as Southern transfer hybridization (STH), dot blot
hybridization (DB) and in situ hybridization (ISH), 2) signal amplified
hybridization assays, such as hybrid capture assays (HC2), 3) target
amplification assays, such as polymerase chain reaction (PCR) and in situ PCR.
In recent years, there has been developed the DNA microarrays for the detection
of HPV. The advantage of PCR-based methods of HPV DNA detection is that they
allow for the identification of different types of HPV. Moreover, PCR-based
detection is both highly sensitive and specific. This study discusses the
advantages and disadvantages of the different methods of HPV DNA detection. In
the future, with the advance of technology, viral DNA extraction and
amplification systems will become more rapid, more sensitive and more
automated.

HPV, detection, assay, molecular, identification

No

0

Yes

122

63

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https://pergamos.lib.uoa.gr/uoa/dl/object/1314242