Unit:
Κατεύθυνση Έρευνα στην Γυναικεία ΑναπαραγωγήLibrary of the School of Health Sciences
Author:
Νικολάου Νικόλαος
Supervisors info:
Μαστοράκος Γεώργιος, Tomlinson Jeremy, Βαλσαμάκης Γεώργιος
Original Title:
Impact of androgens on lipid metabolism
Translated title:
Επίδραση των ανδρογόνων στο μεταβολισμό των λιπιδίων.
Summary:
Non-alcoholic fatty liver disease [NAFLD] has been associated with androgen
deficiency, yet in the majority of patients with non-alcoholic steatohepatitis
[NASH], androgens levels are normal. In contrast, women with polycystic ovarian
syndrome [PCOS], which is characterised by androgen excess, have evidence of
increased liver fat. Our hypothesis is that androgen exposure to the liver may
be crucial in the amount of lipid that can accumulate in hepatocytes. C3A human
hepatoma cells were cultured and treated with Testosterone [Testo] (5nM, 50nM)
or Dihydrotestosterone [DHT] (1nM, 10nM) for 24h. Lipid accumulation was
measured by C14 acetate incorporation and gene expression by real-time PCR.
Cells were transfected with an androgen receptor [AR] construct (pcDNA3.1+AR)
or vector as a control. FASN, ACC1, ACC2 and CPT1 mRNA expression was
significantly increased after dose dependent treatment with Testosterone and
DHT and this was further augmented after AR over. Both Testosterone and DHT
increased de novo lipogenesis and this was also increased following AR over
expression In conclusion, these data highlight that enhanced androgen action is
able to drive lipid accumulation in human hepatocytes and this may be crucial
in understanding the association between PCOS and NAFLD.
Keywords:
Liver, Androgens, Metabolism, NAFLD, PCOS
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