Unit:
Διατμηματικό / Διϊδρυτικό ΠΜΣ Μοριακή ΙατρικήLibrary of the School of Health Sciences
Supervisors info:
Ανάγνου Ν. Ιατρική ΕΚΠΑ
Original Title:
In vitro μελέτη της έκφρασης και του ρόλου συγκεκριμένων long non coding RNΑs στην εξέλιξη της νεφρικής ίνωσης.
Summary:
Chronic kidney disease (CKD) is a frequent condition, causing severe long-term
effects with devastating personal and societal consequences. There is a need for
novel approaches to prevent the decline in renal function during progression of
CKD.
In previous studies of our lab we identified the full transcriptome of renal
tissue,
using the RNA-seq methodology, during early and late time intervals of kidney
fibrosis (unilateral ureteric obstruction (UUO) model). Interestingly, we
noticed a
high number of differentially expressed lncRNAs that could be involved in renal
pathology. To address this question we selected three of them and we performed
transfection experiments in cultured tubular epithelial cells, focusing on a
panel of
fibrosis-related and cell cycle-related genes. We showed that overexpression of
each
lncRNA affects the molecular expression profile of selected fibrosis-related
genes in
a different fashion. Regarding cell cycle-related genes, several genes whose
products
are inhibitors of the cell cycle were significantly up-regulated. This finding
was
further supported by the results from proliferation assays which indicate that
at
least in our cell system, all three lncRNAs lead to reduction of their
proliferative
capacity, with no effect on the ability for apoptosis. Overall, these findings
provide
evidence that the differential expression of these lncRNAs may exert an
important
influence on cellular phenotype during renal process.
Keywords:
Long non coding RNΑs, Kidney fibrosis, Kidney, Overexpression, Tubular epithelial cells
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